Biosample: SAMEA2697775
Protocols: Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA.
Organism:
Scientific Name:
Mus musculus
Sample Accession:
SAMEA2697775
Strain:
129/Ola
Center Name:
Max Plank Institute for Molecular Biomedicine
Sample Alias:
E-MTAB-2684:si_p60_B
Checklist:
ERC000011
Broker Name:
ArrayExpress
Sample Title:
si_p60_B
ENA-CHECKLIST:
ERC000011