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Interaction | |
EBI-8837467 | |
MI Features | |
deg_odph_vhl_1 | |
n-terminus | |
required hydroxypro-402 | |
btb/poz_fold | |
vhl_tumour_suppress_b/a_dom |
Interaction Details
Accession
EBI-8837467Type
Detection Method
Host Organism
In vitroPositive Interaction
✔️External Cross References (1)
Database | Identifier |
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Annotations (7)
Topic | Description |
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imex curation | |
Temp = 25C | |
Accepted 2013-NOV-29 by PPORRAS | |
13-Jan-2014: Contacted by IntAct-Help. | |
Table 1 | |
Ranges for the regions of pVHL and elongin C were taken from PMID:10205047. | |
Kinetic parameters for binding of Hif1a to VHL-elonginB/C complex were determined for different Hif1a peptides. For Hif1a (residues 390-417): kon = 7.9 +/- 0.07 x 10E+5 M-1 s-1, koff = 4.1 +/- 0.03 x 10E-2 s-1 and Kd = 5.2 x 10E-8 molar. For Hif1a (residues 395-406): kon = 4.9 +/- 0.02 x 10E+5 M-1 s-1, koff = 4.5 +/- 0.01 x 10E-2 s-1 and Kd = 9.3 x 10E-8 molar. |
Publication
Title
Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL.Journal
Nature (0028-0836)Authors
Hon W.C.,Wilson M.I.,Harlos K.,Claridge T.D.,Schofield C.J.,Pugh C.W.,Maxwell P.H.,Ratcliffe P.J.,Stuart D.I.,Jones E.Y.Publication date
01/01/2002Publication reference
PubMed: 12050673External Cross References (1)
Database | Identifier |
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Annotations (1)
Topic | Description |
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yvonne@strubi.ox.ac.uk,peter.ratcliffe@ndm.ox.ac.uk |
Showing 1 to 4 of 4 entries
Expand | Name | AC | Type | Identifier | Aliases | Description | Species | Expression System | Detection Methods | Experimental Role | Biological Role | Experimental Preparations | Parameters | Confidences | Cross References | Annotations |
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Expand | Name | AC | Type | Identifier | Aliases | Description | Species | Expression System | Detection Methods | Experimental Role | Biological Role | Experimental Preparations | Parameters | Confidences | Cross References | Annotations |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
elob_human | EBI-8837472 | protein | Elongin-B | Homo sapiens | Escherichia coli | prey | unspecified role | |||||||||
vhl_human | EBI-8837471 | protein | von Hippel-Lindau disease tumor suppressor | Homo sapiens | Escherichia coli | prey | unspecified role |
remark-internal
DIP protein P40337 original sequence version: 175
remark-internal
DIP protein P40337 original sequence version: 160
remark-internal
DIP protein P40337-1 original sequence version: 177
remark-internal
DIP protein P40337 original sequence version: 103
remark-internal
DIP protein P40337 original sequence version: 176
| ||||||||
eloc_human | EBI-8837470 | protein | Elongin-C | Homo sapiens | Escherichia coli | prey | unspecified role | |||||||||
hif1a_human | EBI-8837469 | protein | Hypoxia-inducible factor 1-alpha | Homo sapiens | Chemical synthesis (Chemical synthesis) | bait | unspecified role |
remark-internal
DIP protein Q16665 original sequence version: 177
remark-internal
DIP protein Q16665 original sequence version: 156
remark-internal
DIP protein Q16665 original sequence version: 150
remark-internal
DIP protein Q16665 original sequence version: 179
remark-internal
DIP protein Q16665 original sequence version: 191
remark-internal
DIP protein Q16665-1 original sequence version: 191
remark-internal
DIP protein Q16665-1 original sequence version: 174
remark-internal
DIP protein Q16665 original sequence version: 174
remark-internal
DIP protein Q16665 original sequence version: 169
remark-internal
DIP protein Q16665 original sequence version: 120
remark-internal
DIP protein Q16665 original sequence version: 127
remark-internal
DIP protein Q16665 original sequence version: 102
remark-internal
DIP protein Q16665-1 original sequence version: 102
remark-internal
DIP protein Q16665 original sequence version: 98
remark-internal
DIP protein Q16665 original sequence version: 94
|
Showing 1 to 5 of 5 entries
AC | Name | Type | Role | Range Positions | Linked Features | Participant Name | Participant Identifier | Participant AC | Detection Methods | Parameters | Identifiers | Cross References | Annotations |
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AC | Name | Type | Role | Range Positions | Linked Features | Participant Name | Participant Identifier | Participant AC | Detection Methods | Parameters | Identifiers | Cross References | Annotations |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
EBI-8837482 | vhl_tumour_suppress_b/a_dom | sufficient to bind | 54-213 | vhl_human | EBI-8837471 | ||||||||
EBI-8837479 | btb/poz_fold | sufficient to bind | 17-112 | eloc_human | EBI-8837470 | ||||||||
EBI-8837473 | deg_odph_vhl_1 | sufficient to bind | 395-406 | hif1a_human | EBI-8837469 |
elm
DEG_ODPH_VHL_1
| |||||||
EBI-8837489 | n-terminus | biotin tag | n-n | hif1a_human | EBI-8837469 | ||||||||
EBI-8837476 | required hydroxypro-402 | 4hypro | 402-402 | hif1a_human | EBI-8837469 |
prerequisite-ptm
hydroxylation of pro402
|