Interaction Viewer

  • Collapse
  • Scale:
ComplexViewer 2.2.1by Rappsilber LaboratoryTSG101HGStooltip

Legend

Node Shape
Protein: click to switch
Interaction
EBI-760432

Interaction Details

Accession
EBI-760432
Detection Method
two hybrid pooling
Positive Interaction
✔️
External Cross References (2)
DatabaseIdentifier
imex
psi-mi MI:0469
Annotations (5)
TopicDescription
figure legend supplementary Table S2
accepted Annotation to indicate that this entry has been checked and accepted by a senior curator.
exp-modification All ORFs from the human ORFeome v1.1 (~8,100 ORFs equivalent to ~7200 unique genes) were transfered into DB and AD vectors. Total of 11,210 DB ISTs were crossed against 11,194 AD ISTs to obtain 10,597 Y2H ISTs. This corresponded to 3,703 non-redundant interactions of which 2,754 were high confidence interactions.
curation depth imex curation
data-processing 1) The MGC identifiers provided by authors were used to parse the UniProtKB records to obtain UniProtKB identifiers. 2) The genes that could not be identified were then subject to parsing the gene name and description line of UniProtKB entries using the gene symbol. 3) The sequences for the genes which could not be assigned a UniProtKB identifier were then matched against the entire UniProtKB. 197 clones from the entire ORF could not be mapped using UniProtKB identifiers. Of a total of 2755 interactions 2671 interactions could be mapped using UniProtKB identifiers.

Publication

Title
Towards a proteome-scale map of the human protein-protein interaction network.
Journal
Nature (0028-0836)
Authors
Rual J.F.,Venkatesan K.,Hao T.,Hirozane-Kishikawa T.,Dricot A.,Li N.,Berriz G.F.,Gibbons F.D.,Dreze M.,Ayivi-Guedehoussou N.,Klitgord N.,Simon C.,Boxem M.,Milstein S.,Rosenberg J.,Goldberg D.S.,Zhang L.V.,Wong S.L.,Franklin G.,Li S.,Albala J.S.,Lim J.,Fraughton C.,Llamosas E.,Cevik S.,Bex C.,Lamesch P.,Sikorski R.S.,Vandenhaute J.,Zoghbi H.Y.,Smolyar A.,Bosak S.,Sequerra R.,Doucette-Stamm L.,Cusick M.E.,Hill D.E.,Roth F.P.,Vidal M.
Publication date
01/01/2005
Publication reference
PubMed: 16189514
External Cross References (1)
DatabaseIdentifier
imex
Annotations (2)
TopicDescription
comment This dataset has been named 'Human interactome dataset - co-AP' by the author.
contact-email marc_vidal@dfci.harvard.edu,fritz_roth@hms.harvard.edu,david_hill@dfci.harvard.edu