IntAct European Bioinformatics Institute intact http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom https://www.ebi.ac.uk European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SD, United Kingdom. cblb-crkl-4 Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation. mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom sequence tag sequence tag identification bernardm@ciml.univ-mrs.fr,roncagalli@ciml.univ-mrs.fr imex curation Voisinne G., García-Blesa A., Chaoui K., Fiore F., Bergot E., Girard L., Malissen M., Burlet-Schiltz O., Gonzalez de Peredo A., Malissen B., Roncagalli R. 2016 Molecular systems biology notify when curated Cd4+ cells (purified from pooled lymph nodes and spleens) were were left unstimulated or stimulated for 30, 120, 300, or 600 s with anti‐CD3 and anti‐CD4 antibodies. They were then affinity purified on Strep‐Tactin Sepharose beads and eluted with biotin. No entries have been made for the protein "PCB" as it cannot be uniquely identified. Only proteins given an approximate P(Detection) value of >0.3 have been entered here, as deduced from colour intensity in Figure 2C/D. Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation. Only protein-protein interactions yes ubs3b_mouse Ubiquitin-associated and SH3 domain-containing protein B UBASH3B Tyrosine-protein phosphatase STS1/TULA2 T-cell ubiquitin ligand 2 Suppressor of T-cell receptor signaling 1 Cbl-interacting protein p70 STS1 protein protein mouse Mus musculus Mouse MAAREELYSKVTPRRDRLQRPGTVKHGSALDVLLSMGFPRARAQKALASTGGRSVQAACDWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYSPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTVSSSSLAFGDGALERRQYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTVIDHVYCSPSLRCVQTAHNILKGLQQDNHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPVSKLAISESYDTYINRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE 9B665F45AF208EF6 unspecified role unspecified role prey prey stoichiometry: 0 grap_mouse GRB2-related adapter protein GRAP protein protein mouse Mus musculus Mouse MESVALYSFQATESDELAFNKGDTLKILNMEDDQNWYKAELRGAEGFVPKNYIRVKPHPWYSGRISRQLAEETLMKRNHLGAFLIRESESSPGEFSVSVNYGDQVQHFKVLREASGKYFLWEEKFNSLNELVDFYRTTTIAKRRQIFLCDEQPLIKPSRACFAQAQFDFSAQDPSQLSLRRGDIVEVVEREDPHWWRGRAGGRLGFFPRSYVQPVHL 1AF124C3D2773DD2 unspecified role unspecified role prey prey stoichiometry: 0 grb2_mouse Growth factor receptor-bound protein 2 GRB2 SH2/SH3 adapter GRB2 Adapter protein GRB2 protein protein mouse Mus musculus Mouse MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQMPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 97F4A4FE4B248DDF unspecified role unspecified role prey prey stoichiometry: 0 crkl_mouse Crk-like protein CRKL Crkol protein protein mouse Mus musculus Mouse MSSARFDSSDRSAWYMGPVTRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPVGSVSAPNLPTAEENLEYVRTLYDFPGNDAEDLPFKKGELLVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPTVASTPGAAINPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDDNE A8C801F78EF73573 unspecified role unspecified role prey prey stoichiometry: 0 ubs3a_mouse Ubiquitin-associated and SH3 domain-containing protein A Suppressor of T-cell receptor signaling 2 T-cell ubiquitin ligand 1 Sts2 UBASH3A protein protein mouse Mus musculus Mouse MAAGETQLYAKVSNKLKGRSTPSLLDPLLAMGFPTHTALKALAATGRKTAEAAADWLHGHCNDPSLDDPIPQEYALFLCPTGPLLEKLQEFWRESRRQCAKNRAHEVFPHVTLCDFFTCEDQKVECLYEALRRAGDRILGSFPTLVPLVLHSSISYLGFFINDSPADAIREFAMAFATEAAVLADCTIKPCTKQLHLTLAHKFYPHHQRTLEQLAKAIQPSHSCQWTAALYSRDMRFVHYQTLKALFQYKPQNADELMLSAGDYIFVDPTQQEEASEGWAIGISHRTGCRGFLPENYTERANEADTWVKHRTYTFNLAMDLNSRKDFEASCRGNGEPHTPSMSKSVSSIQALQATISRRGILVVRHGERVDQVFGKSWLQQCTTADGKYYRPDLNFPRSLPRRSNGIKDFENDPPLSSCGIFQARLAGEALLDSGVRVTAVFASPALRCVQTAKHILEELKLEKKLKIRVEPGIFEWMKWEASKATLTFLTLEELKEANFNVDLDYRPALPRCSLMPAESYDQYVERCAVSMGQIINTCPQDMGITLIVSHSSALDSCTRPLLGLPPRECGDFAQLVRKIPSLGMCFCEENREDGKWDLVNPPVKTLTHGANSVFNWRNWISSN E40AFB541AC84538 unspecified role unspecified role prey prey stoichiometry: 0 cblb_mouse E3 ubiquitin-protein ligase CBL-B CBLB RING-type E3 ubiquitin transferase CBL-B Signal transduction protein CBL-B SH3-binding protein CBL-B Casitas B-lineage lymphoma proto-oncogene b protein protein mouse Mus musculus Mouse MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPERPPPIPPDNRLSRHFHHGESVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSSLNGRHSRMGSEQVLMRKHRRHDLPSEGAKVFSNGHLATEEYDVPPRLSPPPPVTTLLPSIKCTGPLANCLSEKTRDTVEDDDDEYKIPSSHPVSLNSQPSHCHNVKAPVRSCDNGHCILNGTHGAPSEMKKSNIPDLGIYLKGGGSDSASDPVPLPPARPPPRDSPKHGSSVNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPTSGQVPLPPARRAAGDSGKANRASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAFPPPVSPRLNL 14B55C4106F84A86 unspecified role unspecified role bait bait OST-modified_strep2_tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range stoichiometry: 0 lax1_mouse Lymphocyte transmembrane adapter 1 LAX Linker for activation of X cells Membrane-associated adapter protein LAX LAX1 protein protein mouse Mus musculus Mouse MYSTPAPPEVTRRNSEPSTRQGTLGSLQGEKGQIIFPGFVVLLTIILVIIAACILWSWKKQKKRPVPYFQVAPSLTLPPPRHRAKNIYDFLPRQQTELGRHQLSGFSTESLLSRASDSPEPEAPQANGSLQMHRVSVHTVEYSVNIYDNGTVPQMCRHLASSTHHVCVRTSRSNPSISSKESNDYVNIPTVEDTCETLTRIESTPENHLGLPSALRLEFAEGGHAGCGNATDHDGFWAPGPKCSDSLSDGDDLSQTSNDYVNMTGLDLENIQENRPRGAFQCCRDYENVPWVDTNESQLPTLEEVASSTVDHREPVWRTLSSVYHMAFQPSAQSEDSAMVHREEQSSEDSSDYETVLVAELEGRDWKQGPGTQHPSDEGTPGDLAGKLCEVVYPAGSLATETSDEDA 00C3F063A7CFC7BE unspecified role unspecified role prey prey stoichiometry: 0 ep15r_mouse Epidermal growth factor receptor substrate 15-like 1 Eps15-related protein Epidermal growth factor receptor pathway substrate 15-related sequence Eps15-rs EPS15R EPS15L1 protein protein mouse Mus musculus Mouse MAAPLVPLSQQIPGGNPLYESYYKQVDPAYTGRVGASEAALFLKKSGLSDIILGKIWDLADPEGKGFLDKQGFYVALRLVACAQSGHEVTLSSLSLTMPPPKFHDTSSPLMATQSSAETHWAVRVEEKAKFDGIFESLLPVNGLLSGDKVKPVLMNSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSILPPPLIPPSKRKKTVFAGAVPVLPASPPPKDSLRSTPSHGSVSSLNSTGSLSPKHSVKQPPVAWVVPVADKMRFDEIFLKTDLDLDGYVSGQEVKEIFMHSGLTQNLLAHIWALADTRQTGKLSKEQFALAMYFIQQKVSKGIDPPQVLSPDMVPPSERGTPIPDSSSTLASGEFTGVKELDDISQEIAQLQREKYSLEQDIREKEEAIRQKTSEVQELQNDLDRETSSLQELEAQKQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQTISSLKTQIQSQESDLKSQEDDLNRAKSELNRLQQEETQLEQSIQAGRAQLETILRSLKCTQDDINQARSKLSQLQESHLEAHRSLEQYDQVPDGVSGTSLPDLATLNEGILLAERGGFGAMDDPFKNKALLFSNNSQELHPDPFQAEDPFKSDPFKGADPFKGDPFQSDPFSEQQTAATDPFGGDPFKESDPFHSSSSDDFFKKQTKNDPFTSDPFTKNPSLPSKLDPFESSDPFSSSSISSKGSDPFGTLDPFGSSSFSSAEGFADFSQMSKPPPSGPFSSSLGGTGFSDDPFKSKQDTPALPPKKPAPPRPKPPSGQSTPVSQLGSSDFPESPDPFQPLGADSGDPFQNKKGFGDPFSGKDPFAPSSSAKPPKTSSSGFADFTSFGNEEQQLAWAKRESEKAEQERLARLRRQEQEDLELAIALSKADMPA B5AF1A89B445E8A7 unspecified role unspecified role prey prey stoichiometry: 0 prs8_mouse 26S proteasome regulatory subunit 8 PSMC5 26S proteasome AAA-ATPase subunit RPT6 p45/SUG Proteasome subunit p45 Proteasome 26S subunit ATPase 5 SUG1 protein protein mouse Mus musculus Mouse MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK 29C6410C4A85A7F7 unspecified role unspecified role prey prey stoichiometry: 0 association association 2C, Dataset Ev1, Sheet B.