F
IPR000821

Alanine racemase

InterPro entry
Short nameAla_racemase
Overlapping
homologous
superfamilies
 
PLP-binding barrel (IPR029066)

Description

Alanine racemase catalyses the pyridoxal-dependent conversion of L-alanine into D-alanine, a key component of bacterial peptidoglycan
[1]
. In Escherichia coli and Salmonella typhimurium, there are two alanine racemase isoforms, alr is a biosynthetic form required for cell wall formation; and dadB functions in L-alanine catabolism. By contrast with dadB and alr, both of which are monomeric enzymes, the alanine racemase of Bacillaceae are homodimers. In Pseudomonas putida, a broad-specificity amino acid racemase is structurally and functionally related to alanine racemase. The 3D-structure of the dimeric alanine racemase from Bacillus stearothermophilus has been determined to a resolution of 1.9 A
[2]
. Each monomer comprises two domains, with an eight-stranded α/β barrel at the N terminus, and a C-terminal β-strand domain. In the dimer, the mouth of the α/β barrel of one monomer faces the second domain of the other monomer. The pyridoxal 5'-phosphate (PLP) cofactor lies in and above the barrel mouth and is covalently linked via an aldimine linkage to Lys39. Several other residues are involved in anchoring the PLP, for example, Arg219 forms a hydrogen bond with the pyridine nitrogen of the cofactor, which is assumed to influence electron delocalisation in PLP-alanine intermediates; Arg136 donates a hydrogen bond to the phenolic oxygen of PLP, and may be involved in substrate binding and stabilisation of intermediates; and Tyr265' is postulated to be a 2 proton donor to the carbanion intermediate
[2]
.

A particular case is represented by Enterococcus VanT, which has both serine and alanine racemase activities, although it is able to racemize serine more efficiently than alanine. Unlike other alanine and serine racemases, VanT is a transmembrane protein. At least ten transmembrane helices are predicted to be present in the N-terminal domain
[4]
and the C-terminal domain has structural homology with the alanine racemase
[3]
and it is matched by this entry.

References

1.Recent topics in pyridoxal 5'-phosphate enzyme studies. Hayashi H, Wada H, Yoshimura T, Esaki N, Soda K. Annu. Rev. Biochem. 59, 87-110, (1990). View articlePMID: 2197992

2.Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution. Shaw JP, Petsko GA, Ringe D. Biochemistry 36, 1329-42, (1997). View articlePMID: 9063881

3.Serine and alanine racemase activities of VanT: a protein necessary for vancomycin resistance in Enterococcus gallinarum BM4174. Arias CA, Weisner J, Blackburn JM, Reynolds PE. Microbiology (Reading, Engl.) 146 ( Pt 7), 1727-34, (2000). PMID: 10878136

4.Characterization and modelling of VanT: a novel, membrane-bound, serine racemase from vancomycin-resistant Enterococcus gallinarum BM4174. Arias CA, Martin-Martinez M, Blundell TL, Arthur M, Courvalin P, Reynolds PE. Mol. Microbiol. 31, 1653-64, (1999). View articlePMID: 10209740

GO terms

cellular component

  • None

Cross References

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