F
IPR000888

dTDP-4-dehydrorhamnose 3,5-epimerase-like

InterPro entry
Short nameRmlC-like
Overlapping
homologous
superfamilies
 

Description

Deoxythymidine diphosphate (dTDP)-4-keto-6-deoxy-d-hexulose 3, 5-epimerase (RmlC,
5.1.3.13
) is involved in the biosynthesis of dTDP-l-rhamnose, which is an essential component of the bacterial cell wall, converting dTDP-4-keto-6-deoxy-D-glucose to dTDP-4-keto-L-rhamnose.

The crystal structure of RmlC from Methanobacterium thermoautotrophicum was determined in the presence and absence of a substrate analogue. RmlC is a homodimer comprising a central jelly roll motif, which extends in two directions into longer β-sheets. Binding of dTDP is stabilised by ionic interactions to the phosphate group and by a combination of ionic and hydrophobic interactions with the base. The active site, which is located in the centre of the jelly roll, is formed by residues that are conserved in all known RmlC sequence homologues. The active site is lined with a number of charged residues and a number of residues with hydrogen-bonding potentials, which together comprise a potential network for substrate binding and catalysis. The active site is also lined with aromatic residues which provide favorable environments for the base moiety of dTDP and potentially for the sugar moiety of the substrate
[1]
.

Also included in this family is the probable dTDP-4-oxo-2,6-dideoxy-D-glucose 3,5-epimerase from Streptomyces antibioticus which is required for the biosynthesis of the aglycone antibiotic oleandomycin
[2]
.

References

1.Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from Methanobacterium thermoautotrophicum complexed with dTDP. Christendat D, Saridakis V, Dharamsi A, Bochkarev A, Pai EF, Arrowsmith CH, Edwards AM. J. Biol. Chem. 275, 24608-12, (2000). View articlePMID: 10827167

2.Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus. Aguirrezabalaga I, Olano C, Allende N, Rodriguez L, Brana AF, Mendez C, Salas JA. Antimicrob. Agents Chemother. 44, 1266-75, (2000). View articlePMID: 10770761

GO terms

biological process

  • None

cellular component

  • None

Cross References

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