IPR001287
Nitrite reductase, copper-type
InterPro entry
Short name | NO2-reductase_Cu |
Overlapping homologous superfamilies | |
family relationships |
Description
These enzymes are involved in nitrate assimilation in the denitrification pathway. The 3D structure of the copper-containing nitrite reductase (NIR) from Achromobacter cycloclastes has been determined to 2.3A resolution
[1]. The enzyme is a trimer, each monomer of which contains two Greek key β-barrel domains (similar to that of plastocyanin) and houses two copper sites. The two copper atoms in the monomer comprise a type I copper site (Cu-I: two His, one Cys and one Met ligand) that plays a crucial role for electron transfer from pseudoazurin to the type II copper site (Cu-II: three His and one solvent ligand), the catalytic centre of NIR for the reduction of nitrite. The Cu-II site lies at the bottom of a 12A deep solvent channel and is the site to which the substrate (NO2-) binds
[1].
References
1.The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes. Godden JW, Turley S, Teller DC, Adman ET, Liu MY, Payne WJ, LeGall J. Science 253, 438-42, (1991). View articlePMID: 1862344
GO terms
biological process
- None
molecular function
cellular component
- None
Cross References
ENZYME
Representative structure
1zv2: Cu-containing nitrite reductase