D
IPR001853

DSBA-like thioredoxin domain

InterPro entry
Short nameDSBA-like_thioredoxin_dom
Overlapping
homologous
superfamilies
 

Description

DSBA is a sub-family of the Thioredoxin family
[1]
. The efficient and correct folding of bacterial disulphide bonded proteinsin vivois dependent upon a class of periplasmic oxidoreductase proteins called DsbA, after the Escherichia coli enzyme. The bacterial protein-folding factor DsbA is the most oxidizing of the thioredoxin family. DsbA catalyses disulphide-bond formation during the folding of secreted proteins. The extremely oxidizing nature of DsbA has been proposed to result from either domain motion or stabilising active-site interactions in the reduced form. DsbA's highly oxidizing nature is a result of hydrogen bond, electrostatic and helix-dipole interactions that favour the thiolate over the disulphide at the active site
[2]
. In the pathogenic bacterium Vibrio cholerae, the DsbA homologue (TcpG) is responsible for the folding, maturation and secretion of virulence factors.

While the overall architecture of TcpG and DsbA is similar and the surface features are retained in TcpG, there are significant differences. For example, the kinked active site helix results from a three-residue loop in DsbA, but is caused by a proline in TcpG (making TcpG more similar to thioredoxin in this respect). Furthermore, the proposed peptide binding groove of TcpG is substantially shortened compared with that of DsbA due to a six-residue deletion. Also, the hydrophobic pocket of TcpG is more shallow and the acidic patch is much less extensive than that of E. coli DsbA
[1]
.

References

1.Structure of TcpG, the DsbA protein folding catalyst from Vibrio cholerae. Hu SH, Peek JA, Rattigan E, Taylor RK, Martin JL. J. Mol. Biol. 268, 137-46, (1997). View articlePMID: 9149147

2.Crystal structures of reduced and oxidized DsbA: investigation of domain motion and thiolate stabilization. Guddat LW, Bardwell JC, Martin JL. Structure 6, 757-67, (1998). View articlePMID: 9655827

GO terms

biological process

  • None

cellular component

  • None

Cross References

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