D
IPR002223

Pancreatic trypsin inhibitor Kunitz domain

InterPro entry
Short nameKunitz_BPTI
Overlapping
homologous
superfamilies
 

Description

The majority of the sequences having this domain belong to the MEROPS inhibitor family I2, clan IB; the Kunitz/bovine pancreatic trypsin inhibitor family, they inhibit proteases of the S1 family
[6]
and are restricted to the metazoa with a single exception: Amsacta moorei entomopoxvirus. They are short (~50 residue) α/β proteins with few secondary structures. The fold is constrained by 3 disulphide bonds. The type example for this family is aprotinin (bovine pancreatic trypsin inhibitor)
[1]
(or basic protease inhibitor), but the family includes numerous other members
[2, 3, 4, 5]
, such as snake venom basic protease; mammalian inter-alpha-trypsin inhibitors; trypstatin, a rodent mast cell inhibitor of trypsin; a domain found in an alternatively-spliced form of Alzheimer's amyloid beta-protein; domains at the C-termini of the alpha(1) and alpha(3) chains of type VII and type VI collagens; and tissue factor pathway inhibitor precursor.

The pancreatic trypsin inhibitor (Kunitz) family
[7, 2, 3]
is one of the numerous families of serine proteinase inhibitors. The basic structure of such a type of inhibitor is shown in the following schematic representation:

            +-----------------------+
            |  +--------+           |
            |  |      **|*******    |
          xxCxxC#xxxCxxxCxxxxxxCxxxxCxx
                    |          |
                    +----------+

            {------50 residues------}

'C': conserved cysteine involved in a disulfide bond.
'#': active site residue.
'*': position of the pattern.

References

1.Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor. Housset D, Kim KS, Fuchs J, Woodward C, Wlodawer A. J. Mol. Biol. 220, 757-70, (1991). View articlePMID: 1714504

2.Inter-alpha-trypsin inhibitor: emergence of a family within the Kunitz-type protease inhibitor superfamily. Salier JP. Trends Biochem. Sci. 15, 435-9, (1990). View articlePMID: 1703675

3.Evolutionary origin of a Kunitz-type trypsin inhibitor domain inserted in the amyloid beta precursor protein of Alzheimer's disease. Ikeo K, Takahashi K, Gojobori T. J. Mol. Evol. 34, 536-43, (1992). View articlePMID: 1593645

4.Molecular cloning, expression, and partial characterization of a second human tissue-factor-pathway inhibitor. Sprecher CA, Kisiel W, Mathewes S, Foster DC. Proc. Natl. Acad. Sci. U.S.A. 91, 3353-7, (1994). View articlePMID: 8159751

5.Amino acid sequence of a protease inhibitor isolated from Sarcophaga bullata determined by mass spectrometry. Papayannopoulos IA, Biemann K. Protein Sci. 1, 278-88, (1992). View articlePMID: 1304909

6.Evolutionary families of peptidase inhibitors. Rawlings ND, Tolle DP, Barrett AJ. Biochem. J. 378, 705-16, (2004). View articlePMID: 14705960

7.Protein inhibitors of proteinases. Laskowski M Jr, Kato I. Annu. Rev. Biochem. 49, 593-626, (1980). View articlePMID: 6996568

GO terms

biological process

  • None

cellular component

  • None

Cross References

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