F
IPR002637

RdgB/HAM1

InterPro entry
Short nameRdgB/HAM1
Overlapping
homologous
superfamilies
 
family relationships

Description

This family contains a group of purine NTP pyrophosphatases, including dITP/XTP pyrophosphatase RdgB from E.coli and inosine triphosphate pyrophosphatase (ITPA, also known as HAM1) from Saccharomyces cerevisiae (Baker's yeast) (
P47119
), as well as archaeal and Caenorhabditis elegans homologues. S. cerevisiae HAM1 was originally identified as a gene that protects against the mutagenic effects of the base analogue 6-N-hydroxylaminopurine (HAP) which can be a natural product of monooxygenase activity on adenine
[1, 3]
. Subsequent studies demonstrated that HAM1 detoxifies abnormal pyrimidine as well as purine nucleotides
[2]
. RdgB appears to act by intercepting non-canonical deoxyribonucleotide triphosphates from replication precursor pools
[5, 6, 7]
.

Similarly, a Ham1-related protein from Methanococcus jannaschii is a novel NTPase that has been shown to hydrolyze nonstandard nucleotides such as XTP to XMP and ITP to IMP, but not the standard nucleotides, in the presence of Mg or Mn ions. The enzyme exists as a homodimer
[4]
.

References

1.HAM1, the gene controlling 6-N-hydroxylaminopurine sensitivity and mutagenesis in the yeast Saccharomyces cerevisiae. Noskov VN, Staak K, Shcherbakova PV, Kozmin SG, Negishi K, Ono BC, Hayatsu H, Pavlov YI. Yeast 12, 17-29, (1996). View articlePMID: 8789257

2.Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae. Takayama S, Fujii M, Kurosawa A, Adachi N, Ayusawa D. Curr. Genet. 52, 203-11, (2007). View articlePMID: 17899088

3.Overexpression of the yeast HAM1 gene prevents 6-N-hydroxylaminopurine mutagenesis in Escherichia coli. Kozmin SG, Leroy P, Pavlov YI. Acta Biochim. Pol. 45, 645-52, (1998). PMID: 9918490

4.Structure-based identification of a novel NTPase from Methanococcus jannaschii. Hwang KY, Chung JH, Kim SH, Han YS, Cho Y. Nat. Struct. Biol. 6, 691-6, (1999). View articlePMID: 10404228

5.Identification of the dITP- and XTP-hydrolyzing protein from Escherichia coli. Chung JH, Park HY, Lee JH, Jang Y. J Biochem Mol Biol 35, 403-8, (2002). PMID: 12297000

6.Molecular basis of the antimutagenic activity of the house-cleaning inosine triphosphate pyrophosphatase RdgB from Escherichia coli. Savchenko A, Proudfoot M, Skarina T, Singer A, Litvinova O, Sanishvili R, Brown G, Chirgadze N, Yakunin AF. J. Mol. Biol. 374, 1091-103, (2007). View articlePMID: 17976651

7.Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase. Burgis NE, Cunningham RP. J. Biol. Chem. 282, 3531-8, (2007). View articlePMID: 17090528

GO terms

Cross References

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