D
IPR002735

Translation initiation factor IF2/IF5 domain

InterPro entry
Short nameTransl_init_fac_IF2/IF5_dom
Overlapping
homologous
superfamilies
 

Description

The beta subunit of archaeal and eukaryotic translation initiation factor 2 (IF2beta) and the N-terminal domain of translation initiation factor 5 (IF5) show significant sequence homology
[1]
. Archaeal IF2beta contains two independent structural domains: an N-terminal mixed α/β core domain (topological similarity to the common core of ribosomal proteins L23 and L15e), and a C-terminal domain consisting of a zinc-binding C4 finger
[2]
. Archaeal IF2beta is a ribosome-dependent GTPase that stimulates the binding of initiator Met-tRNA(i)(Met) to the ribosomes, even in the absence of other factors
[3]
. The C-terminal domain of eukaryotic IF5 is involved in the formation of the multi-factor complex (MFC), an important intermediate for the 43S pre-initiation complex assembly. IF5 interacts directly with IF1, IF2beta and IF3c, which together with IF2-bound Met-tRNA(i)(Met) form the MFC
[4]
.

This entry represents both the N-terminal and zinc-binding domains of IF2, as well as a domain in IF5.

References

1.Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: a representative of the eIF2beta/eIF5 family of proteins. Cho S, Hoffman DW. Biochemistry 41, 5730-42, (2002). View articlePMID: 11980477

2.Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation. Gutierrez P, Osborne MJ, Siddiqui N, Trempe JF, Arrowsmith C, Gehring K. Protein Sci. 13, 659-67, (2004). View articlePMID: 14978306

3.Functional analysis of the translation factor aIF2/5B in the thermophilic archaeon Sulfolobus solfataricus. Maone E, Di Stefano M, Berardi A, Benelli D, Marzi S, La Teana A, Londei P. Mol. Microbiol. 65, 700-13, (2007). View articlePMID: 17608795

4.The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5. Bieniossek C, Schutz P, Bumann M, Limacher A, Uson I, Baumann U. J. Mol. Biol. 360, 457-65, (2006). View articlePMID: 16781736

GO terms

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