D
IPR002810

NfeD-like, C-terminal domain

InterPro entry
Short nameNfeD-like_C
Overlapping
homologous
superfamilies
 

Description

NfeD-like proteins are widely distributed throughout prokaryotes and are frequently associated with genes encoding stomatin-like proteins (slipins)
[2]
. The homologue from hyperthermophilic archaebacterium Pyrococcus horikoshii is thought to be a serine protease involved in ion channel opening through the cleavage of a stomatin-homologue
[2]
.

There appear to be three major groups of NfeD-like proteins: an ancestral group with only an N-terminal serine protease domain and this C-terminal β- sheet-rich domain which is structurally very similar to the OB-fold domain
[3]
, associated with its neighbouring slipin cluster; a second major group with an additional middle, membrane-spanning domain, associated in some species with eoslipin and in others with yqfA; a final 'artificial' group which unites truncated forms lacking the protease region and associated with their ancestral gene partner, either yqfA or eoslipin.

This NefD, C-terminal domain appears to be the major one for relating to the associated protein. NfeD homologues are clearly reliant on their conserved gene neighbour which is assumed to be necessary for function, either through direct physical interaction or by functioning in the same pathway, possibly involve with lipid-rafts
[1]
.

References

1.The NfeD protein family and its conserved gene neighbours throughout prokaryotes: functional implications for stomatin-like proteins. Green JB, Lower RP, Young JP. J. Mol. Evol. 69, 657-67, (2009). View articlePMID: 20012272

2.A novel thermostable membrane protease forming an operon with a stomatin homolog from the hyperthermophilic archaebacterium Pyrococcus horikoshii. Yokoyama H, Matsui I. J. Biol. Chem. 280, 6588-94, (2005). View articlePMID: 15611110

3.The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold. Kuwahara Y, Ohno A, Morii T, Yokoyama H, Matsui I, Tochio H, Shirakawa M, Hiroaki H. Protein Sci. 17, 1915-24, (2008). View articlePMID: 18687870

Cross References

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