F
IPR003724

ATP:cob(I)alamin adenosyltransferase CobA/CobO/BtuR

InterPro entry
Short nameCblAdoTrfase_CobA
Overlapping
homologous
superfamilies
 

Description

ATP:cob(I)alamin (or ATP:corrinoid) adenosyltransferases (
2.5.1.17
), catalyse the conversion of cobalamin (vitamin B12) into its coenzyme form, adenosylcobalamin (AdoCbl)or coenzyme B12
[1]
. AdoCbl contains an adenosyl moiety liganded to the cobalt ion of cobalamin via a covalent Co-C bond. AdoCbl is required as a cofactor for the activity of certain enzymes.

ATP:cob(I)alamin adenosyltransferases are classed into three groups: CobA-type
[2]
, EutT-type
[3]
and PduO-type
[4]
. Each of the three enzyme types appears to be specialised for particular AdoCbl-dependent enzymes or for the de novo synthesis AdoCbl. PduO and EutT are distantly related, sharing short conserved motifs, while CobA is evolutionarily unrelated and is an example of convergent evolution.

This entry represents the ATP:cob(I)alamin adenosyltransferases CobA (Salmonella typhimurium), CobO (Pseudomonas aeruginosa), and BtuR (Escherichia coli). There is a high degree of sequence identity between these proteins
[5]
. CobA is a homodimer responsible for attaching the adenosyl moiety from ATP to the cobalt ion of the corrin ring, necessary for the conversion of cobalamin to adenosylcobalamin
[2, 1, 6, 7]
.

References

1.Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica. Sheppard DE, Penrod JT, Bobik T, Kofoid E, Roth JR. J. Bacteriol. 186, 7635-44, (2004). View articlePMID: 15516577

2.Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1. Buan NR, Rehfeld K, Escalante-Semerena JC. J. Bacteriol. 188, 3543-50, (2006). View articlePMID: 16672609

3.The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme. Buan NR, Suh SJ, Escalante-Semerena JC. J. Bacteriol. 186, 5708-14, (2004). View articlePMID: 15317775

4.Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene. Johnson CL, Pechonick E, Park SD, Havemann GD, Leal NA, Bobik TA. J. Bacteriol. 183, 1577-84, (2001). View articlePMID: 11160088

5.Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium. Suh SJ, Escalante-Semerena JC. Gene 129, 93-7, (1993). View articlePMID: 7916712

6.Biosynthesis of cobalamin (vitamin B(12)). Scott AI, Roessner CA. Biochem. Soc. Trans. 30, 613-20, (2002). View articlePMID: 12196148

7.The biosynthesis of adenosylcobalamin (vitamin B12). Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC. Nat Prod Rep 19, 390-412, (2002). View articlePMID: 12195810

GO terms

Cross References

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