D
IPR006483

CRISPR-associated Cas3-type, HD domain

InterPro entry
Short nameCRISPR-assoc_Cas3_HD
Overlapping
homologous
superfamilies
 

Description

This entry represents the HD domain, which is found in a number of Cas proteins that tend to be found near CRISPR repeats. These domains can be found either separately or as the N-terminal region of Cas3, the helicase-containing CRISPR-associated protein. CRISPR loci appear to be mobile elements with a wide host range. The Cas3-type HD domain has nuclease activity against ssDNA and ssRNA
[2, 1]
.

The CRISPR-Cas system is a prokaryotic defense mechanism against foreign genetic elements. The key elements of this defense system are the Cas proteins and the CRISPR RNA.

References

1.Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference. Beloglazova N, Petit P, Flick R, Brown G, Savchenko A, Yakunin AF. EMBO J. 30, 4616-27, (2011). View articlePMID: 22009198

2.Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. Sinkunas T, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. EMBO J. 30, 1335-42, (2011). View articlePMID: 21343909

Further reading

3. Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein. Howard JA, Delmas S, Ivancic-Bace I, Bolt EL. Biochem. J. 439, 85-95, (2011). View articlePMID: 21699496

4. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. Biol. Direct 1, 7, (2006). View articlePMID: 16545108

5. CRISPR provides acquired resistance against viruses in prokaryotes. Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P. Science 315, 1709-12, (2007). View articlePMID: 17379808

6. Evolutionary conservation of sequence and secondary structures in CRISPR repeats. Kunin V, Sorek R, Hugenholtz P. Genome Biol. 8, R61, (2007). View articlePMID: 17442114

7. A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii. Brendel J, Stoll B, Lange SJ, Sharma K, Lenz C, Stachler AE, Maier LK, Richter H, Nickel L, Schmitz RA, Randau L, Allers T, Urlaub H, Backofen R, Marchfelder A. J. Biol. Chem. 289, 7164-77, (2014). View articlePMID: 24459147

Cross References

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