D
IPR006642

Rad18, zinc finger UBZ4-type

InterPro entry
Short nameRad18_UBZ4
domain relationships

Description

The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. UBZ domains coordinate a zinc ion with cysteine or histidine residues; depending on their amino acid sequence, UBZ domains are classified into several families
[8, 3]
. Type 1 UBZs are CCHH-type zinc fingers found in tandem UBZ domains of TAX1-binding protein 1 (TAX1BP1)
[10, 5, 14]
, type 2 UBZs are CCHC-type zinc fingers found in FAAP20 which is a subunit of the Fanconi anemia (FA) core complex
[6, 12]
, type 3 UBZs are CCHH-type zinc fingers found only in the Y-family translesion polymerase eta
[4, 9, 11]
, and type 4 UBZs are CCHC-type zinc fingers found in Y-family translesion polymerase kappa, Werner helicase-interacting protein 1 (WRNIP1), and Rad18
[13, 7, 15]
.

This entry represents type 4 UBZ found in RAD18. The domain is a potential zinc finger for nucleic acid binding and a putative nucleotide binding sequence
[2]
. Human RAD18 accumulates very rapidly and remains for a long period of time at sites of different types of DNA damage, and is required of DNA. RAD18 appears to respond to DNA damage in two distinct ways: replication-dependent and replication-independent. The RAD18-type zinc finger located in the middle of RAD18 is responsible for the replication-independent accumulation of RAD18 following DNA damage, while a second zinc finger, SAP-type, is responsible for replication-dependent accumulation
[1]
.

References

1.Replication-dependent and -independent responses of RAD18 to DNA damage in human cells. Nakajima S, Lan L, Kanno S, Usami N, Kobayashi K, Mori M, Shiomi T, Yasui A. J. Biol. Chem. 281, 34687-95, (2006). View articlePMID: 16980296

2.The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence. Jones JS, Weber S, Prakash L. Nucleic Acids Res. 16, 7119-31, (1988). View articlePMID: 2970061

3.A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1. Suzuki N, Rohaim A, Kato R, Dikic I, Wakatsuki S, Kawasaki M. FEBS J. 283, 2004-17, (2016). View articlePMID: 27062441

4.Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta. Bomar MG, Pai MT, Tzeng SR, Li SS, Zhou P. EMBO Rep. 8, 247-51, (2007). View articlePMID: 17304240

5.Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2. Xie X, Li F, Wang Y, Wang Y, Lin Z, Cheng X, Liu J, Chen C, Pan L. Autophagy 11, 1775-89, (2015). View articlePMID: 26506893

6.Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain. Wojtaszek JL, Wang S, Kim H, Wu Q, D'Andrea AD, Zhou P. Nucleic Acids Res. 42, 13997-4005, (2014). PMID: 25414354

7.NMR structure of the human Rad18 zinc finger in complex with ubiquitin defines a class of UBZ domains in proteins linked to the DNA damage response. Rizzo AA, Salerno PE, Bezsonova I, Korzhnev DM. Biochemistry 53, 5895-906, (2014). PMID: 25162118

8.Ubiquitin-binding domains and their role in the DNA damage response. Hofmann K. DNA Repair (Amst.) 8, 544-56, (2009). PMID: 19213613

9.The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication. Garcia-Ortiz MV, Roldan-Arjona T, Ariza RR. FEBS J. 274, 3340-50, (2007). PMID: 17550419

10.The structure of TAX1BP1 UBZ1+2 provides insight into target specificity and adaptability. Ceregido MA, Spinola Amilibia M, Buts L, Rivera-Torres J, Garcia-Pino A, Bravo J, van Nuland NA. J. Mol. Biol. 426, 674-90, (2014). PMID: 24239949

11.The unusual UBZ domain of Saccharomyces cerevisiae polymerase ?. Woodruff RV, Bomar MG, D'Souza S, Zhou P, Walker GC. DNA Repair (Amst.) 9, 1130-41, (2010). PMID: 20837403

12.Structural basis for ubiquitin recognition by ubiquitin-binding zinc finger of FAAP20. Toma A, Takahashi TS, Sato Y, Yamagata A, Goto-Ito S, Nakada S, Fukuto A, Horikoshi Y, Tashiro S, Fukai S. PLoS ONE 10, e0120887, (2015). PMID: 25799058

13.RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger. Yang K, Moldovan GL, D'Andrea AD. J. Biol. Chem. 285, 19085-91, (2010). PMID: 20385554

14.Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy. Thurston TL, Boyle KB, Allen M, Ravenhill BJ, Karpiyevich M, Bloor S, Kaul A, Noad J, Foeglein A, Matthews SA, Komander D, Bycroft M, Randow F. EMBO J. 35, 1779-92, (2016). PMID: 27370208

15.Distinct functional roles for the two SLX4 ubiquitin-binding UBZ domains mutated in Fanconi anemia. Lachaud C, Castor D, Hain K, Munoz I, Wilson J, MacArtney TJ, Schindler D, Rouse J. J. Cell. Sci. 127, 2811-7, (2014). PMID: 24794496

GO terms

biological process

molecular function

cellular component

  • None

Cross References

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