F
IPR006754

Poxvirus I3 ssDNA-binding

InterPro entry
Short namePoxvirus_I3_ssDNA-bd

Description

The 34kDa protein encoded by the I3 gene of vaccinia virus, named Protein OPG079, is expressed at early and intermediate times post-infection and is phosphorylated on serine residues. I3 protein demonstrates a striking affinity for single-stranded, but not for double-stranded, DNA. Electrophoretic mobility shift assays indicate that numerous I3 molecules can bind to a template, reflecting the stoichiometric interaction of I3 with DNA. Sequence analysis reveals that a pattern of aromatic and charged amino acids common to many replicative single-stranded DNA binding proteins (SSBs) is conserved in I3
[1]
. This protein plays an essential role in viral DNA replication
[2]
.

References

1.Characterization of the single-stranded DNA binding protein encoded by the vaccinia virus I3 gene. Rochester SC, Traktman P. J. Virol. 72, 2917-26, (1998). View articlePMID: 9525612

2.The acidic C-terminus of vaccinia virus I3 single-strand binding protein promotes proper assembly of DNA-protein complexes. Harrison ML, Desaulniers MA, Noyce RS, Evans DH. Virology 489, 212-22, (2016). PMID: 26773382

GO terms

biological process

  • None

cellular component

  • None
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