IPR008332
Methylguanine DNA methyltransferase, ribonuclease-like domain
InterPro entry
Short name | MethylG_MeTrfase_N |
Overlapping homologous superfamilies |
Description
The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (
2.1.1.63) (also known as 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase). The major mutagenic and carcinogenic effect of methylating agents in DNA is the formation of O6-alkylguanine. The alkyl group at the O-6 position is transferred to a cysteine residue in the enzyme
[1]. This is a suicide reaction since the enzyme is irreversibly inactivated and the methylated protein accumulates as a dead-end product. Most, but not all of the methyltransferases are also able to repair O-4-methylthymine. DNA-[protein]-cysteine S-methyltransferases are widely distributed and are found in various prokaryotic and eukaryotic sources
[2].
This group of proteins are characterised by having an N-terminal ribonuclease-like domain, represented by this entry, that is associated with 6-O-methylguanine DNA methyltransferase activity (
IPR001497).
References
1.Regulation and expression of the adaptive response to alkylating agents. Lindahl T, Sedgwick B, Sekiguchi M, Nakabeppu Y. Annu. Rev. Biochem. 57, 133-57, (1988). View articlePMID: 3052269
2.Isolation and partial characterisation of a Chinese hamster O6-alkylguanine-DNA alkyltransferase cDNA. Rafferty JA, Elder RH, Watson AJ, Cawkwell L, Potter PM, Margison GP. Nucleic Acids Res. 20, 1891-5, (1992). View articlePMID: 1579490
GO terms
biological process
cellular component
- None
Cross References
ENZYME
Contributing Member Database Entry
- Pfam:PF02870