D
IPR008332

Methylguanine DNA methyltransferase, ribonuclease-like domain

InterPro entry
Short nameMethylG_MeTrfase_N
Overlapping
homologous
superfamilies
 

Description

The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (
2.1.1.63
) (also known as 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase). The major mutagenic and carcinogenic effect of methylating agents in DNA is the formation of O6-alkylguanine. The alkyl group at the O-6 position is transferred to a cysteine residue in the enzyme
[1]
. This is a suicide reaction since the enzyme is irreversibly inactivated and the methylated protein accumulates as a dead-end product. Most, but not all of the methyltransferases are also able to repair O-4-methylthymine. DNA-[protein]-cysteine S-methyltransferases are widely distributed and are found in various prokaryotic and eukaryotic sources
[2]
.

This group of proteins are characterised by having an N-terminal ribonuclease-like domain, represented by this entry, that is associated with 6-O-methylguanine DNA methyltransferase activity (
IPR001497
).

References

1.Regulation and expression of the adaptive response to alkylating agents. Lindahl T, Sedgwick B, Sekiguchi M, Nakabeppu Y. Annu. Rev. Biochem. 57, 133-57, (1988). View articlePMID: 3052269

2.Isolation and partial characterisation of a Chinese hamster O6-alkylguanine-DNA alkyltransferase cDNA. Rafferty JA, Elder RH, Watson AJ, Cawkwell L, Potter PM, Margison GP. Nucleic Acids Res. 20, 1891-5, (1992). View articlePMID: 1579490

GO terms

Cross References

Contributing Member Database Entry
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.