F
IPR008367

Regucalcin

InterPro entry
Short nameRegucalcin
Overlapping
homologous
superfamilies
 
family relationships

Description

Regucalcin, also known as senesence marker protein-30 (SMP30), was discovered in 1978 as a Ca2+binding protein that does not contain EF-hand motifs, suggesting a novel class of Ca2+binding protein. It is primarily localised to the liver and kidney cortex of animals. Expression of its mRNA in the liver and renal cortex of rats is stimulated by an increase in cellular Ca2+levels
[7, 3]
.

Regucalin, as a regulatory protein of Ca2+, has a pivotal role in the control of many cell functions. The protein has a reversible effect on Ca2+-induced activation and inhibition of many enzymes in both the liver and renal cortex cells
[1]
. It has also been shown to inhibit various protein kinases (including Ca2+/calmodulin-dependent protein kinase
[4]
, protein kinase C
[5]
and tyrosine kinase) and protein phosphatases, indicating a regulatory role in signal transduction within the cell. In addition, regucalcin regulates intracellular Ca2+homeostasis by enhancing Ca2+-pumping activity in the plasma membrane through activation of the pump enzymes
[2]
. Moreover, it can inhibit RNA synthesis in the nuclei of normal and regenerating rat livers in vitro
[6]
.

Hydropathy profiles indicate hydrophobic domains in both N-and C-terminal regions of the regucalcin molecule; the protein also exhibits hydrophilic characteristics
[1]
. Human and rodent regucalcins share 89% sequence identity, the high degree of conservation between species suggesting that the complete structure is required for physiological function.

References

1.Reversible effect of calcium-binding protein regucalcin on the Ca(2+)-induced inhibition of deoxyuridine 5'-triphosphatase activity in rat liver cytosol. Yamaguchi M, Sakurai T. Mol. Cell. Biochem. 110, 25-9, (1992). View articlePMID: 1315924

2.Regulatory effect of regucalcin on (Ca(2+)-Mg2+)-ATPase in rat liver plasma membranes: comparison with the activation by Mn2+ and Co2+. Takahashi H, Yamaguchi M. Mol. Cell. Biochem. 124, 169-74, (1993). View articlePMID: 8232287

3.Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. Shimokawa N, Yamaguchi M. FEBS Lett. 305, 151-4, (1992). View articlePMID: 1618342

4.Hepatic calcium-binding protein regucalcin decreases Ca2+/calmodulin-dependent protein kinase activity in rat liver cytosol. Mori S, Yamaguchi M. Chem. Pharm. Bull. 38, 2216-8, (1990). PMID: 2177680

5.Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat renal cortex cytosol. Kurota H, Yamaguchi M. Biol. Pharm. Bull. 21, 315-8, (1998). PMID: 9586564

6.Inhibitory effect of calcium-binding protein regucalcin on ribonucleic acid synthesis in isolated rat liver nuclei. Yamaguchi M, Ueoka S. Mol. Cell. Biochem. 173, 169-75, (1997). View articlePMID: 9278268

7.Molecular cloning and sequencing of the cDNA coding for a calcium-binding protein regucalcin from rat liver. Shimokawa N, Yamaguchi M. FEBS Lett. 327, 251-5, (1993). View articlePMID: 8348951

GO terms

biological process

  • None

cellular component

  • None

Cross References

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