F
IPR008380

HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase

InterPro entry
Short nameHAD-SF_hydro_IG_5-nucl
Overlapping
homologous
superfamilies
 
family relationships

Description

This family includes a 5'-nucleotidase,
3.1.3.5
, specific for purines (IMP and GMP)
[1]
. These enzymes are members of the Haloacid Dehalogenase (HAD) superfamily. HAD members are recognised by three short motifs {hhhhDxDx(T/V)}, {hhhh(T/S)}, and either {hhhh(D/E)(D/E)x(3-4)(G/N)} or {hhhh(G/N)(D/E)x(3-4)(D/E)} (where "h" stands for a hydrophobic residue). Crystal structures of many HAD enzymes has verified PSI-PRED predictions of secondary structural elements which show each of the "hhhh" sequences of the motifs as part of β sheets. This subfamily of enzymes is part of "Subfamily I" of the HAD superfamily by virtue of a "cap" domain in between motifs 1 and 2. This subfamily's cap domain has a different predicted secondary structure than all other known HAD enzymes and thus has been designated "subfamily IG", the domain appears to consist of a mixed α/β fold.

References

1.Bovine cytosolic IMP/GMP-specific 5'-nucleotidase: cloning and expression of active enzyme in Escherichia coli. Allegrini S, Pesi R, Tozzi MG, Fiol CJ, Johnson RB, Eriksson S. Biochem. J. 328 ( Pt 2), 483-7, (1997). View articlePMID: 9371705

Cross References

Contributing Member Database Entries
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