IPR010992
Integration host factor (IHF)-like DNA-binding domain superfamily
InterPro entry
Short name | IHF-like_DNA-bd_dom_sf |
Overlapping entries |
Description
Integration host factor (IHF) (
IPR005684,
IPR005685) is a small heterodimeric protein that binds the minor groove of DNA in a sequence-specific manner and induces a large bend. This bending stabilises distinct DNA conformations that are required during several bacterial processes, such as recombination, transposition, replication and transcription
[1]. The core structure of IHF consists of a partly opened 4-helical bundle that is capped with a β-sheet.
Prokaryotic protein HU and the bacteriophage SPO1 transcription factor TF1 are closely related to IHF. These proteins are collectively referred to as type II DNA-binding proteins (DBPII), forming a group of basic, dimeric proteins found in all bacteria that are able to bind DNA to induce and stabilise DNA bending. HU plays a structural role in replication initiation, transcription regulation, site-specific recombination, and the compaction of the bacterial genome
[2]. TF1 is essential for viral multiplication
[3].
References
1.Integration host factor: putting a twist on protein-DNA recognition. Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA. J. Mol. Biol. 330, 493-502, (2003). View articlePMID: 12842466
2.Flexible DNA bending in HU-DNA cocrystal structures. Swinger KK, Lemberg KM, Zhang Y, Rice PA. EMBO J. 22, 3749-60, (2003). View articlePMID: 12853489
3.Solution structure of a mutant of transcription factor 1: implications for enhanced DNA binding. Liu W, Vu HM, Geiduschek EP, Kearns DR. J. Mol. Biol. 302, 821-30, (2000). View articlePMID: 10993726
4.Solution structure of the DNA-binding domain of TraM. Stockner T, Plugariu C, Koraimann G, Hogenauer G, Bermel W, Prytulla S, Sterk H. Biochemistry 40, 3370-7, (2001). View articlePMID: 11258958
GO terms
Cross References
ENZYME
Contributing Member Database Entries
- CATH-Gene3D:G3DSA:4.10.520.10
- SUPERFAMILY:SSF47729
Representative structure
4p3v: Crystal structure of the E. coli HU beta2 protein