H
IPR011324

Cytotoxic necrotizing factor-like, catalytic

InterPro entry
Short nameCytotoxic_necrot_fac-like_cat
Overlapping entries
 

Description

This entry represents the catalytic domain from several bacterial cytotoxic necrotizing factor proteins and the related dermonecrotic toxin (DNT) from Bordetella species. Cytotoxic necrotizing factor 1 (CNF1) is a toxin which in Escherichia coli forms a 4-layer α/β/β/α structure containing mixed β-sheets
[1]
. CNF1 is expressed in strains of E. coli causing uropathogenic and neonatal meningitis. It alters the host cell actin cytoskeleton and promotes bacterial invasion of the blood-brain barrier endothelial cells
[2]
. CNF1 belongs to a unique group of large cytotoxins that cause constitutive activation of Rho guanosine triphosphatases (GTPases), which are key regulators of the actin cytoskeleton
[1]
.

Bordetella dermonecrotic toxin (DNT) stimulates the assembly of actin stress fibres and focal adhesions by deamidating or polyaminating Gln63 of the small GTPase Rho. DNT is an A-B toxin composed of an N-terminal receptor-binding (B) domain and a C-terminal enzymatically active (A) domain
[3]
.

A similar structural motif can be found in several hypothetical proteins, including YfiH from Shigella flexneri
[4]
, YlmD from Bacillus stearothermophilus, NMB0706 from Neisseria meningitidis serogroup B and CC0490 from Caulobacter crescentus.

References

1.Structure of the Rho-activating domain of Escherichia coli cytotoxic necrotizing factor 1. Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P. Nat. Struct. Biol. 8, 584-8, (2001). View articlePMID: 11427886

2.Expression of cnf1 by Escherichia coli J96 involves a large upstream DNA region including the hlyCABD operon, and is regulated by the RfaH protein. Landraud L, Gibert M, Popoff MR, Boquet P, Gauthier M. Mol. Microbiol. 47, 1653-67, (2003). View articlePMID: 12622819

3.Identification of a receptor-binding domain of Bordetella dermonecrotic toxin. Matsuzawa T, Kashimoto T, Katahira J, Horiguchi Y. Infect. Immun. 70, 3427-32, (2002). View articlePMID: 12065482

4.Crystal structure of hypothetical protein YfiH from Shigella flexneri at 2 A resolution. Kim Y, Maltseva N, Dementieva I, Collart F, Holzle D, Joachimiak A. Proteins 63, 1097-101, (2006). View articlePMID: 16498617

Cross References

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