D
IPR012480

Heparinase II/III-like, C-terminal

InterPro entry
Short nameHepar_II_III_C

Description

This is the C-terminal domain of heparin and heparin-sulfate lyase (HepB, also known as heparinase II), heparin-sulfate lyase (HepC, also known as heparinase III) and distantly related sequences. The structures of heparinase II and III have been solved
[3, 2]
.

HepB cleaves both heparin and heparan sulfate glycosaminoglycans through a beta-elimination mechanism. It cleaves heparin at alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S and heparan sulfate at alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp
[1]
. HepC specifically cleaves heparan sulfate-rich regions of acidic polysaccharides, it does not act on N,O-desulfated glucosamine or N-acetyl-O-sulfated glucosamine linkages. HepC functions in cleaving metazoan heparan sulfate and providing carbon, nitrogen and sulfate sources for microorganisms
[4]
.

This entry also includes FtsZ-localized protein C (FzlC), which binds and recruits FtsZ polymers to membranes early in the cell cycle. It may also improve the efficiency of cytokinesis through the regulation of cell wall hydrolysis. This protein has been reported to have sequence similarity to heparinase II/III family proteins
[5]
.

References

1.Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum. Su H, Blain F, Musil RA, Zimmermann JJ, Gu K, Bennett DC. Appl. Environ. Microbiol. 62, 2723-34, (1996). View articlePMID: 8702264

2.Structural basis of heparan sulfate-specific degradation by heparinase III. Dong W, Lu W, McKeehan WL, Luo Y, Ye S. Protein Cell 3, 950-61, (2012). View articlePMID: 23011846

3.Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product. Shaya D, Tocilj A, Li Y, Myette J, Venkataraman G, Sasisekharan R, Cygler M. J. Biol. Chem. 281, 15525-35, (2006). View articlePMID: 16565082

4.Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III. Pojasek K, Shriver Z, Hu Y, Sasisekharan R. Biochemistry 39, 4012-9, (2000). View articlePMID: 10747789

5.A novel membrane anchor for FtsZ is linked to cell wall hydrolysis in Caulobacter crescentus. Meier EL, Razavi S, Inoue T, Goley ED. Mol Microbiol 101, 265-80, (2016). View articlePMID: 27028265

GO terms

biological process

  • None

molecular function

cellular component

  • None

Cross References

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