F
IPR012734

Dihydroxyacetone kinase

InterPro entry
Short nameDhaK_ATP

Description

This family consists of examples of the single chain form of dihydroxyacetone kinase (also called glycerone kinase) that uses ATP (
2.7.1.29
) as the phosphate donor, rather than a phosphoprotein as in Escherichia coli. This form has separable domains homologous to the K and L subunits of the E. coli enzyme, and is found in yeasts and other eukaryotes and in some bacteria, including Citrobacter freundii
[2, 3]
.

The member from tomato has been shown to phosphorylate dihydroxyacetone, 3,4-dihydroxy-2-butanone, and some other aldoses and ketoses
[1]
. Members from mammals have been shown to catalyse both the phosphorylation of dihydroxyacetone and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate
[4]
. In yeast there are two isozymes of dihydroxyacetone kinase (Dak1 and Dak2). They are required for detoxification of dihydroxyacetone (DHA)
[5]
.

References

1.A tomato enzyme catalyzing the phosphorylation of 3,4-dihydroxy-2-butanone. Herz S, Kis K, Bacher A, Rohdich F. Phytochemistry 60, 3-11, (2002). View articlePMID: 11985845

2.Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii. Daniel R, Stuertz K, Gottschalk G. J. Bacteriol. 177, 4392-401, (1995). PMID: 7635824

3.Crystal structure of the Citrobacter freundii dihydroxyacetone kinase reveals an eight-stranded alpha-helical barrel ATP-binding domain. Siebold C, Arnold I, Garcia-Alles LF, Baumann U, Erni B. J. Biol. Chem. 278, 48236-44, (2003). View articlePMID: 12966101

4.Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases. Cabezas A, Costas MJ, Pinto RM, Couto A, Cameselle JC. Biochem. Biophys. Res. Commun. 338, 1682-9, (2005). View articlePMID: 16289032

5.Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production. Itoh N, Tujibata Y, Liu JQ. Appl. Microbiol. Biotechnol. 51, 193-200, (1999). View articlePMID: 10091325

GO terms

Cross References

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