H
IPR014777

Tetrapyrrole methylase, subdomain 1

InterPro entry
Short name4pyrrole_Mease_sub1
Overlapping entries
 
YabN, N-terminal (IPR035013)

Description

Tetrapyrroles are large macrocyclic compounds derived from a common biosynthetic pathway
[1]
. The end-product, uroporphyrinogen III, is used to synthesise a number of important molecules, including cobalamin (vitamin B12), haem, sirohaem, chlorophyll, coenzyme F430 and phytochromobilin
[2]
.

This entry represents the N-terminal subdomain 1 from several tetrapyrrole methylases, which consist of two non-similar domains. These enzymes catalyse the methylation of their substrates using S-adenosyl-L-methionine as a methyl source. Enzymes in this family include:


 * Uroporphyrinogen III methyltransferase (
2.1.1.107
) (SUMT), which catalyses the conversion of uroporphyrinogen III to precorrin-2 at the first branch-point of the tetrapyrrole synthesis pathway, directing the pathway towards cobalamin or sirohaem synthesis
[3]
.
 * Precorrin-2 C20-methyltransferase CobI/CbiL (
2.1.1.130
), which introduces a methyl group at C-20 on precorrin-2 to produce precorrin-3A during cobalamin biosynthesis. This reaction is key to the conversion of a porphyrin-type tetrapyrrole ring to a corrin ring
[4]
. In some species, this enzyme is part of a bifunctional protein.
 * Precorrin-4 C11-methyltransferase CobM/CbiF (
2.1.1.133
), which introduces a methyl group at C-11 on precorrin-4 to produce precorrin-5 during cobalamin biosynthesis
[5]
.
 * Sirohaem synthase CysG (
2.1.1.107
), domains 4 and 5, which synthesizes sirohaem from uroporphyrinogen III, at the first branch-point in the tetrapyrrole biosynthetic pathway, directing the pathway towards sirohaem synthesis
[6]
.
 * Diphthine synthase (
2.1.1.98
), which carries out the methylation step during the modification of a specific histidine residue of elongation factor 2 (EF-2) during diphthine synthesis.

References

1.Biosynthesis of cobalamin (vitamin B12): a bacterial conundrum. Raux E, Schubert HL, Warren MJ. Cell. Mol. Life Sci. 57, 1880-93, (2000). View articlePMID: 11215515

2.Tetrapyrrole biosynthesis in higher plants. Tanaka R, Tanaka A. 58, 321-46, (2007). View articlePMID: 17227226

3.Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis. Vevodova J, Graham RM, Raux E, Schubert HL, Roper DI, Brindley AA, Ian Scott A, Roessner CA, Stamford NP, Elizabeth Stroupe M, Getzoff ED, Warren MJ, Wilson KS. J. Mol. Biol. 344, 419-33, (2004). View articlePMID: 15522295

4.Crystal structures of CbiL, a methyltransferase involved in anaerobic vitamin B biosynthesis, and CbiL in complex with S-adenosylhomocysteine--implications for the reaction mechanism. Wada K, Harada J, Yaeda Y, Tamiaki H, Oh-Oka H, Fukuyama K. FEBS J. 274, 563-73, (2007). View articlePMID: 17229157

5.Genetically engineered synthesis and structural characterization of cobalt-precorrin 5A and -5B, two new intermediates on the anaerobic pathway to vitamin B12: definition of the roles of the CbiF and CbiG enzymes. Kajiwara Y, Santander PJ, Roessner CA, Perez LM, Scott AI. J. Am. Chem. Soc. 128, 9971-8, (2006). View articlePMID: 16866557

6.CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis. Stroupe ME, Leech HK, Daniels DS, Warren MJ, Getzoff ED. Nat. Struct. Biol. 10, 1064-73, (2003). View articlePMID: 14595395

GO terms

biological process

  • None

cellular component

  • None

Cross References

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