D
IPR016188

PurM-like, N-terminal domain

InterPro entry
Short namePurM-like_N
Overlapping
homologous
superfamilies
 

Description

This entry represents a structural domain with a core structure consisting of β-α-β-α-β(2), which is found in two enzymes of the purine biosynthetic pathway: at the N-terminal of aminoimidazole ribonucleotide (AIR) synthetase (PurM)
[1]
, as well as the N1 and N2 domains of formylglycinamide ribonucleotide (FGAR) amidotransferase (PurL) (PurM-like module)
[2]
. PurM and PurL utilise ATP to activate the oxygen of an amide within their substrate toward nucleophilic attack by a nitrogen. PurM uses the product of PurL, formylglycinamidine ribonucleotide (FGAM) and ATP to make AIR, ADP and P(i). It is also found as domains 1 and 3 in phosphoribosylformylglycinamidine synthase II (smPurL) (
6.3.5.3
) (carries a duplication: tandem repeats of two PurM-like units arranged like the PurM subunits in the dimer)
[3]
.

This domain is also found at the N-terminal of thiamine monophosphate kinase (
2.7.4.16
) (ThiL)
[4]
. ThiL phosphorylates thiamine monophosphate to form thiamine pyrophosphate, an essential cofactor that is synthesized de novo by Salmonella typhimurium.

References

1.X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5 A resolution. Li C, Kappock TJ, Stubbe J, Weaver TM, Ealick SE. Structure 7, 1155-66, (1999). View articlePMID: 10508786

2.Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography. Anand R, Hoskins AA, Stubbe J, Ealick SE. Biochemistry 43, 10328-42, (2004). View articlePMID: 15301531

3.Crystal structure of phosphoribosylformylglycinamidine synthase II (smPurL) from Thermotoga maritima at 2.15 A resolution. Mathews II, Krishna SS, Schwarzenbacher R, McMullan D, Abdubek P, Ambing E, Canaves JM, Chiu HJ, Deacon AM, DiDonato M, Elsliger MA, Godzik A, Grittini C, Grzechnik SK, Hale J, Hampton E, Han GW, Haugen J, Jaroszewski L, Klock HE, Koesema E, Kreusch A, Kuhn P, Lesley SA, Levin I, Miller MD, Moy K, Nigoghossian E, Paulsen J, Quijano K, Reyes R, Spraggon G, Stevens RC, van den Bedem H, Velasquez J, White A, Wolf G, Xu Q, Hodgson KO, Wooley J, Wilson IA. Proteins 63, 1106-11, (2006). View articlePMID: 16544324

4.Characterization of thiL, encoding thiamin-monophosphate kinase, in Salmonella typhimurium. Webb E, Downs D. J. Biol. Chem. 272, 15702-7, (1997). View articlePMID: 9188462

Cross References

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