F
IPR016435

Diphthamide synthesis DPH1/DPH2

InterPro entry
Short nameDPH1/DPH2
Overlapping
homologous
superfamilies
 
family relationships

Description

Archaeal and eukaryotic translation elongation factor 2 contain a unique posttranslationally modified histidine residue called diphthamide, the target of the diphtheria toxin. Diphtheria toxin inhibits eukaryotic protein synthesis by ADP-ribosylating diphthamide in EF2
[1]
.

Members of this family include 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1/2 (also known as Diphtheria toxin resistance protein 1/2, DPH 1/2), which are involved in the first step of diphthamide synthesis
[1, 3]
. Archaeal DPHs are more similar to eukaryotic DPH1 than to DPH2
[2]
.

References

1.Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Liu S, Milne GT, Kuremsky JG, Fink GR, Leppla SH. Mol. Cell. Biol. 24, 9487-97, (2004). View articlePMID: 15485916

2.Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme. Zhang Y, Zhu X, Torelli AT, Lee M, Dzikovski B, Koralewski RM, Wang E, Freed J, Krebs C, Ealick SE, Lin H. Nature 465, 891-6, (2010). View articlePMID: 20559380

3.The asymmetric function of Dph1-Dph2 heterodimer in diphthamide biosynthesis. Dong M, Dando EE, Kotliar I, Su X, Dzikovski B, Freed JH, Lin H. J Biol Inorg Chem 24, 777-782, (2019). PMID: 31463593

GO terms

Cross References

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