S
IPR018087

Glycoside hydrolase, family 5, conserved site

InterPro entry
Short nameGlyco_hydro_5_CS

Description

O-Glycosyl hydrolases (
3.2.1.
) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families
[5, 1]
. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glycoside hydrolase family 5
GH5
comprises enzymes with several known activities; endoglucanase (
3.2.1.4
); beta-mannanase (
3.2.1.78
); exo-1,3-glucanase (
3.2.1.58
); endo-1,6-glucanase (
3.2.1.75
); xylanase (
3.2.1.8
); endoglycoceramidase (
3.2.1.123
).

The microbial degradation of cellulose and xylans requires several types of enzymes. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family A
[3]
or as the glycosyl hydrolases family 5
[2]
. One of the conserved regions in this family contains a conserved glutamic acid residue which is potentially involved
[4]
in the catalytic mechanism.

References

1.Structures and mechanisms of glycosyl hydrolases. Davies G, Henrissat B. Structure 3, 853-9, (1995). View articlePMID: 8535779

2.A classification of glycosyl hydrolases based on amino acid sequence similarities. Henrissat B. Biochem. J. 280 ( Pt 2), 309-16, (1991). View articlePMID: 1747104

3.Cellulase families revealed by hydrophobic cluster analysis. Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon JP. Gene 81, 83-95, (1989). View articlePMID: 2806912

4.Cellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues for catalysis. Py B, Bortoli-German I, Haiech J, Chippaux M, Barras F. Protein Eng. 4, 325-33, (1991). View articlePMID: 1677466

5.Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. Proc. Natl. Acad. Sci. U.S.A. 92, 7090-4, (1995). View articlePMID: 7624375

GO terms

Cross References

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