F
IPR022450

tRNA N6-adenosine threonylcarbamoyltransferase, TsaD

InterPro entry
Short nameTsaD
Overlapping
homologous
superfamilies
 
family relationships

Description

This entry represents the TsaD protein family that is widely distributed. TsaD and its archaeal homologue Kae1 belong to the Kae1/TsaD family (
IPR017861
), a conserved protein family with unknown function.

This entry includes bacterial TsaD and its homologues, such as Qri7 (localize to the mitochondria) from budding yeast
[6]
.

TsaD (also known as Gcp or YgjD) was originally described as a glycoprotease essential for cell viability
[7]
and a critical mediator involved in the modification of cell wall peptidoglycan synthesis and/or cell division
[8]
. Gcp is a member of the Kae1/TsaD family, required for the formation of a threonylcarbamoyl group on adenosine at position 37 in tRNAs that read codons beginning with adenine
[6]
. YgjD has been renamed as TsaD, and it has been shown that YgjD and proteins YrdC (TsaC), YjeE (TsaE), and YeaZ (TsaB), are necessary and sufficient for t6A biosynthesis in vitro, and may constitute a complex
[5]
.

The first characterised member of the Kae1/TsaD family was annotated as Gcp for O-sialoglycoprotein endopeptidase
[9]
, but this activity could not be confirmed
[1]
. Later, its homologue, Kae1 from Pyrococcus abyssi, has been shown to have DNA-binding properties and apurinic-endonuclease activity
[1]
. Members of this family have since been studied in yeast, archaea and bacteria resulting in sometimes conflicting data, several proposed functions and annotations but no definitive characterisation. For instance, some members have been linked to DNA maintenance in bacteria and mitochondria
[4]
and transcription regulation and telomere homeostasis in eukaryotes
[2, 3]
, but their function remained unclear. Recent research indicates that this family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine, a universal modification found at position 37 of tRNAs that read codons beginning with adenine
[6, 10]
.

References

1.An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro. Hecker A, Leulliot N, Gadelle D, Graille M, Justome A, Dorlet P, Brochier C, Quevillon-Cheruel S, Le Cam E, van Tilbeurgh H, Forterre P. Nucleic Acids Res. 35, 6042-51, (2007). View articlePMID: 17766251

2.Yeast homolog of a cancer-testis antigen defines a new transcription complex. Kisseleva-Romanova E, Lopreiato R, Baudin-Baillieu A, Rousselle JC, Ilan L, Hofmann K, Namane A, Mann C, Libri D. EMBO J. 25, 3576-85, (2006). View articlePMID: 16874308

3.A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, Guillard S, Partington M, Zubko MK, Krogan NJ, Emili A, Greenblatt JF, Harrington L, Lydall D, Durocher D. Cell 124, 1155-68, (2006). View articlePMID: 16564010

4.Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Oberto J, Breuil N, Hecker A, Farina F, Brochier-Armanet C, Culetto E, Forterre P. Nucleic Acids Res. 37, 5343-52, (2009). View articlePMID: 19578062

5.Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside. Deutsch C, El Yacoubi B, de Crecy-Lagard V, Iwata-Reuyl D. J. Biol. Chem. 287, 13666-73, (2012). View articlePMID: 22378793

6.A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification. El Yacoubi B, Hatin I, Deutsch C, Kahveci T, Rousset JP, Iwata-Reuyl D, Murzin AG, de Crecy-Lagard V. EMBO J. 30, 882-93, (2011). View articlePMID: 21285948

7.Identification of an essential glycoprotease in Staphylococcus aureus. Zheng L, Yang J, Landwehr C, Fan F, Ji Y. FEMS Microbiol. Lett. 245, 279-85, (2005). View articlePMID: 15837383

8.Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Zheng L, Yu C, Bayles K, Lasa I, Ji Y. J. Bacteriol. 189, 2734-42, (2007). View articlePMID: 17237169

9.Cloning, nucleotide sequence, and expression of the Pasteurella haemolytica A1 glycoprotease gene. Abdullah KM, Lo RY, Mellors A. J. Bacteriol. 173, 5597-603, (1991). View articlePMID: 1885539

10.Molecular basis for t6A modification in human mitochondria. Zhou JB, Wang Y, Zeng QY, Meng SX, Wang ED, Zhou XL. Nucleic Acids Res 48, 3181-3194, (2020). PMID: 32047918

GO terms

molecular function

  • None

cellular component

  • None

Cross References

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