D
IPR022649

Proliferating cell nuclear antigen, PCNA, C-terminal

InterPro entry
Short namePr_cel_nuc_antig_C
Overlapping
homologous
superfamilies
 

Description

Proliferating cell nuclear antigen (PCNA), or cyclin, is a non-histone acidic nuclear protein
[4]
that plays a key role in the control of eukaryotic DNA replication
[5]
. It acts as a co-factor for DNA polymerase delta, which is responsible for leading strand DNA replication
[7]
. The sequence of PCNA is well conserved between plants and animals, indicating a strong selective pressure for structure conservation, and suggesting that this type of DNA replication mechanism is conserved throughout eukaryotes
[6]
. In Saccharomyces cerevisiae (Baker's yeast), POL30, is associated with polymerase III, the yeast analog of polymerase delta.

Homologues of PCNA have also been identified in the archaea (known as DNA polymerase sliding clamp)
[3, 2]
and in Paramecium bursaria Chlorella virus 1 (PBCV-1) and in nuclear polyhedrosis viruses.

The N-terminal and C-terminal domains of PCNA are topologically identical. Three PCNA molecules are tightly associated to form a closed ring encircling duplex DNA
[1]
.

References

1.Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA. Krishna TS, Kong XP, Gary S, Burgers PM, Kuriyan J. Cell 79, 1233-43, (1994). View articlePMID: 8001157

2.Two DNA polymerase sliding clamps from the thermophilic archaeon Sulfolobus solfataricus. De Felice M, Sensen CW, Charlebois RL, Rossi M, Pisani FM. J. Mol. Biol. 291, 47-57, (1999). View articlePMID: 10438605

3.Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea. Cann IK, Ishino S, Hayashi I, Komori K, Toh H, Morikawa K, Ishino Y. J. Bacteriol. 181, 6591-9, (1999). PMID: 10542158

4.Molecular cloning of cDNA coding for rat proliferating cell nuclear antigen (PCNA)/cyclin. Matsumoto K, Moriuchi T, Koji T, Nakane PK. EMBO J. 6, 637-42, (1987). View articlePMID: 2884104

5.Identification of carrot cDNA clones encoding a second putative proliferating cell-nuclear antigen, DNA polymerase delta auxiliary protein. Hata S, Kouchi H, Tanaka Y, Minami E, Matsumoto T, Suzuka I, Hashimoto J. Eur. J. Biochem. 203, 367-71, (1992). View articlePMID: 1346518

6.Highly conserved structure of proliferating cell nuclear antigen (DNA polymerase delta auxiliary protein) gene in plants. Suzuka I, Hata S, Matsuoka M, Kosugi S, Hashimoto J. Eur. J. Biochem. 195, 571-5, (1991). View articlePMID: 1671766

7.Structure of the human gene for the proliferating cell nuclear antigen. Travali S, Ku DH, Rizzo MG, Ottavio L, Baserga R, Calabretta B. J. Biol. Chem. 264, 7466-72, (1989). View articlePMID: 2565339

GO terms

molecular function

cellular component

  • None
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