IPR023535
Threonylcarbamoyl-AMP synthase
InterPro entry
Short name | TC-AMP_synthase |
Overlapping homologous superfamilies |
Description
This entry represents the threonylcarbamoyl-AMP synthase, includding RimN or YrdC (also known as TsaC)
[2], which is involved in the biosynthesis of N6-threonyl-carbamoyl-adenosine (t6A) modified tRNA. This is a universal modification, found at position 37 of ANN decoding tRNAs. Analysis shows that YrdC binds ATP and preferentially binds tRNA-Thr lacking the t6A modification
[1]. Furthermore YrdC has been shown to have threonine-dependent ATPase activity that generates AMP
[2]. YrdC (TsaC) and proteins YgjD (TsaD), YjeE (TsaE), and YeaZ (TsaB), are necessary and sufficient for t6A biosynthesis in vitro, and may constitute a complex.
References
1.The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA. El Yacoubi B, Lyons B, Cruz Y, Reddy R, Nordin B, Agnelli F, Williamson JR, Schimmel P, Swairjo MA, de Crecy-Lagard V. Nucleic Acids Res. 37, 2894-909, (2009). View articlePMID: 19287007
2.Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside. Deutsch C, El Yacoubi B, de Crecy-Lagard V, Iwata-Reuyl D. J. Biol. Chem. 287, 13666-73, (2012). View articlePMID: 22378793
GO terms
biological process
molecular function
cellular component
- None
Cross References
ENZYME
Contributing Member Database Entry
- HAMAP:MF_01852
Representative structure
1hru: THE STRUCTURE OF THE YRDC GENE PRODUCT FROM E.COLI