F
IPR023535

Threonylcarbamoyl-AMP synthase

InterPro entry
Short nameTC-AMP_synthase
Overlapping
homologous
superfamilies
 

Description

This entry represents the threonylcarbamoyl-AMP synthase, includding RimN or YrdC (also known as TsaC)
[2]
, which is involved in the biosynthesis of N6-threonyl-carbamoyl-adenosine (t6A) modified tRNA. This is a universal modification, found at position 37 of ANN decoding tRNAs. Analysis shows that YrdC binds ATP and preferentially binds tRNA-Thr lacking the t6A modification
[1]
. Furthermore YrdC has been shown to have threonine-dependent ATPase activity that generates AMP
[2]
. YrdC (TsaC) and proteins YgjD (TsaD), YjeE (TsaE), and YeaZ (TsaB), are necessary and sufficient for t6A biosynthesis in vitro, and may constitute a complex.

References

1.The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA. El Yacoubi B, Lyons B, Cruz Y, Reddy R, Nordin B, Agnelli F, Williamson JR, Schimmel P, Swairjo MA, de Crecy-Lagard V. Nucleic Acids Res. 37, 2894-909, (2009). View articlePMID: 19287007

2.Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside. Deutsch C, El Yacoubi B, de Crecy-Lagard V, Iwata-Reuyl D. J. Biol. Chem. 287, 13666-73, (2012). View articlePMID: 22378793

GO terms

molecular function

cellular component

  • None

Cross References

Contributing Member Database Entry
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