F
IPR024909

Cysteinyl-tRNA synthetase/mycothiol ligase

InterPro entry
Short nameCys-tRNA/MSH_ligase
Overlapping
homologous
superfamilies
 
family relationships

Description

This entry represents a subgroup of the class-I aminoacyl-tRNA synthetase family. It includes cysteinyl-tRNA synthetase and the related enzyme mycothiol ligase
[2]
.

Cysteine-tRNA ligase (also known as Cysteinyl-tRNA synthetase) (
6.1.1.16
) is an alpha monomer and belongs to class Ia
[3]
. It is highly specific despite not possessing the amino acid editing activity characteristic of many other tRNA ligases
[4]
.

Mycothiol ligase, also known as L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, uses ATP to ligate a Cys residue to a mycothiol precursor molecule, in the second to last step in mycothiol biosynthesis
[1]
.

References

1.A coupled spectrophotometric assay for l-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase and its application for inhibitor screening. Newton GL, Ta P, Sareen D, Fahey RC. Anal. Biochem. 353, 167-73, (2006). View articlePMID: 16674910

2.ATP-dependent L-cysteine:1D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, mycothiol biosynthesis enzyme MshC, is related to class I cysteinyl-tRNA synthetases. Sareen D, Steffek M, Newton GL, Fahey RC. Biochemistry 41, 6885-90, (2002). View articlePMID: 12033919

3.Sequence determination and modeling of structural motifs for the smallest monomeric aminoacyl-tRNA synthetase. Hou YM, Shiba K, Mottes C, Schimmel P. Proc. Natl. Acad. Sci. U.S.A. 88, 976-80, (1991). View articlePMID: 1992490

4.Structural origins of amino acid selection without editing by cysteinyl-tRNA synthetase. Newberry KJ, Hou YM, Perona JJ. EMBO J. 21, 2778-87, (2002). View articlePMID: 12032090

Cross References

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