F
IPR027328

Microtubule-associated protein RP/EB

InterPro entry
Short nameMAPRE
Overlapping
homologous
superfamilies
 

Description

This entry represents the microtubule-associated protein RP/EB (MAPRE) family, including MAPRE1 (EB1), MAPRE2 (RP1, also known as EB2), MAPRE3 (EBF3, also known as EB3) and their homologues from eukaryotes. Despite their high protein sequence conservation, the individual EBs exhibit different regulatory and functional properties
[5]
. For instance, EB1 and EB3, but not EB2, promote persistent microtubule growth by suppressing catastrophes
[6]
.

EB1 contains an N-terminal calponin homology (CH) domain that is responsible for the interaction with microtubules (MTs), and a C-terminal coiled coil domain that extends into a four-helix bundle, required for dimer formation
[3]
. Through their C-terminal sequences, EBs interact with most other known +TIPs (plus end tracking proteins) and recruit many of them to the growing MT ends
[2, 3]
. EB1 is involved in MT anchoring at the centrosome and cell migration
[4]
.

EB2 is highly expressed in pancreatic cancer cells, and seems to be involved in perineural invasion
[1]
.

References

1.The microtubule-associated protein MAPRE2 is involved in perineural invasion of pancreatic cancer cells. Abiatari I, Gillen S, DeOliveira T, Klose T, Bo K, Giese NA, Friess H, Kleeff J. Int. J. Oncol. 35, 1111-6, (2009). PMID: 19787265

2.Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Askham JM, Vaughan KT, Goodson HV, Morrison EE. Mol. Biol. Cell 13, 3627-45, (2002). View articlePMID: 12388762

3.Tracking the ends: a dynamic protein network controls the fate of microtubule tips. Akhmanova A, Steinmetz MO. Nat. Rev. Mol. Cell Biol. 9, 309-22, (2008). View articlePMID: 18322465

4.EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration. Wen Y, Eng CH, Schmoranzer J, Cabrera-Poch N, Morris EJ, Chen M, Wallar BJ, Alberts AS, Gundersen GG. Nat. Cell Biol. 6, 820-30, (2004). View articlePMID: 15311282

5.Molecular insights into mammalian end-binding protein heterodimerization. De Groot CO, Jelesarov I, Damberger FF, Bjelic S, Scharer MA, Bhavesh NS, Grigoriev I, Buey RM, Wuthrich K, Capitani G, Akhmanova A, Steinmetz MO. J. Biol. Chem. 285, 5802-14, (2010). View articlePMID: 20008324

6.Mammalian end binding proteins control persistent microtubule growth. Komarova Y, De Groot CO, Grigoriev I, Gouveia SM, Munteanu EL, Schober JM, Honnappa S, Buey RM, Hoogenraad CC, Dogterom M, Borisy GG, Steinmetz MO, Akhmanova A. J. Cell Biol. 184, 691-706, (2009). View articlePMID: 19255245

Further reading

7. EB3 regulates microtubule dynamics at the cell cortex and is required for myoblast elongation and fusion. Straube A, Merdes A. Curr. Biol. 17, 1318-25, (2007). View articlePMID: 17658256

8. Aspergillus fumigatus AcuM regulates both iron acquisition and gluconeogenesis. Liu H, Gravelat FN, Chiang LY, Chen D, Vanier G, Ejzykowicz DE, Ibrahim AS, Nierman WC, Sheppard DC, Filler SG. Mol. Microbiol. 78, 1038-54, (2010). View articlePMID: 21062375

GO terms

biological process

  • None

cellular component

  • None
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