F
IPR028614

GDP-L-fucose synthase/GDP-L-colitose synthase

InterPro entry
Short nameGDP_fucose/colitose_synth
Overlapping
homologous
superfamilies
 

Description

GDP-L-fucose synthase is responsible for the last step of the major metabolic pathway resulting in GDP-L-fucose synthesis from GDP-D-mannose. It catalyses a combined epimerase and NADPH-dependent reductase reaction, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose
[1]
. Since several cell-surface antigens, including the leukocyte Lewis system and cell-surface antigens in pathogenic bacteria, depend on the availability of GDP-L-fucose for their expression, the enzyme is a potential target for therapy in pathological states depending on selectin-mediated cell-to-cell interactions
[2]
.

This entry also includes ColC from Yersinia pseudotuberculosis. ColC is a GDP-L-colitose synthase, which is a bifunctional enzyme catalysaing the C-5 epimerization of GDP-4-keto-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose
[3]
.

References

1.Synthesis of GDP-L-fucose by the human FX protein. Tonetti M, Sturla L, Bisso A, Benatti U, De Flora A. J. Biol. Chem. 271, 27274-9, (1996). View articlePMID: 8910301

2.Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose Epimerase/Reductase by kinetic and crystallographic characterization of site-specific mutants. Rosano C, Bisso A, Izzo G, Tonetti M, Sturla L, De Flora A, Bolognesi M. J. Mol. Biol. 303, 77-91, (2000). View articlePMID: 11021971

3.Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC). Alam J, Beyer N, Liu HW. Biochemistry 43, 16450-60, (2004). View articlePMID: 15610039

GO terms

cellular component

  • None

Cross References

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