F
IPR028878

Ribonuclease E

InterPro entry
Short nameRNase_E
family relationships

Description

Ribonuclease E (RNase E) is an endoribonuclease that plays a central role in RNA processing and decay. It is required for the maturation of 5S and 16S rRNAs, and the majority of tRNAs. It is also involved in the degradation of most mRNAs
[6, 3, 4, 5]
. It is a major subunit of the eubacterial degradosome - a large multiprotein complex involved in RNA processing and degradation in E. coli and other proteobacteria
[2]
. It is required to initiate rRNA degradation during both starvation and quality control; acts after RNase PH (rph) exonucleolytically digests the 3'-end of the 16S rRNA
[7]
. Degradation of 16S rRNA leads to 23S rRNA degradation. RNase E is a multidomain protein containing an amino-terminal catalytic domain, a central RNA-binding domain, and carboxy-terminal binding sites for the other major components of the RNA degradosome
[1]
.

References

1.Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome. Vanzo NF, Li YS, Py B, Blum E, Higgins CF, Raynal LC, Krisch HM, Carpousis AJ. Genes Dev. 12, 2770-81, (1998). View articlePMID: 9732274

2.The RNA degradosome: life in the fast lane of adaptive molecular evolution. Marcaida MJ, DePristo MA, Chandran V, Carpousis AJ, Luisi BF. Trends Biochem. Sci. 31, 359-65, (2006). View articlePMID: 16766188

3.RNase E plays an essential role in the maturation of Escherichia coli tRNA precursors. Li Z, Deutscher MP. RNA 8, 97-109, (2002). View articlePMID: 11871663

4.Cleavage of poly(A) tails on the 3'-end of RNA by ribonuclease E of Escherichia coli. Walsh AP, Tock MR, Mallen MH, Kaberdin VR, von Gabain A, McDowall KJ. Nucleic Acids Res. 29, 1864-71, (2001). View articlePMID: 11328869

5.RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA. Li Z, Pandit S, Deutscher MP. EMBO J. 18, 2878-85, (1999). View articlePMID: 10329633

6.RNase E, an endoribonuclease, has a general role in the chemical decay of Escherichia coli mRNA: evidence that rne and ams are the same genetic locus. Mudd EA, Krisch HM, Higgins CF. Mol. Microbiol. 4, 2127-35, (1990). View articlePMID: 1708438

7.Elucidation of pathways of ribosomal RNA degradation: an essential role for RNase E. Sulthana S, Basturea GN, Deutscher MP. RNA 22, 1163-71, (2016). PMID: 27298395

GO terms

biological process

cellular component

  • None

Cross References

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