F
IPR033309

Crossover junction endonuclease Mus81

InterPro entry
Short nameMus81

Description

Budding yeast Mus81 complexes with Mms4 (EME1 or EME2 in humans) to form a structure-specific endonuclease that targets branched DNA structures with a 5'-end at the branch nick
[1, 4]
. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions
[3, 2]
.

Similar to MUS81-EME1, human MUS81-EME2 cleaves 3'-flaps, replication forks and nicked Holliday junctions, and exhibits limited endonuclease activity with intact Holliday junctions. However, MUS81-EME2 can also cleave a variety of DNA structures including D-loop recombination intermediates and nicked duplex
[5, 6]
.

References

1.Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. Fu Y, Xiao W. DNA Repair (Amst.) 2, 1435-47, (2003). View articlePMID: 14642571

2.Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates? Gaskell LJ, Osman F, Gilbert RJ, Whitby MC. EMBO J. 26, 1891-901, (2007). View articlePMID: 17363897

3.DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Mayle R, Campbell IM, Beck CR, Yu Y, Wilson M, Shaw CA, Bjergbaek L, Lupski JR, Ira G. Science 349, 742-7, (2015). View articlePMID: 26273056

4.Human MUS81 complexes stimulate flap endonuclease 1. Shin YK, Amangyeld T, Nguyen TA, Munashingha PR, Seo YS. FEBS J. 279, 2412-30, (2012). View articlePMID: 22551069

5.Substrate specificity of the MUS81-EME2 structure selective endonuclease. Pepe A, West SC. Nucleic Acids Res. 42, 3833-45, (2014). View articlePMID: 24371268

6.Human MUS81-EME2 can cleave a variety of DNA structures including intact Holliday junction and nicked duplex. Amangyeld T, Shin YK, Lee M, Kwon B, Seo YS. Nucleic Acids Res. 42, 5846-62, (2014). View articlePMID: 24692662

GO terms

Cross References

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