IPR034641
Polysaccharide lyase family 11
InterPro entry
Short name | RGL11 |
Description
The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction
[1, 2]. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
References
1.Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. Ochiai A, Itoh T, Kawamata A, Hashimoto W, Murata K. Appl. Environ. Microbiol. 73, 3803-13, (2007). View articlePMID: 17449691
2.Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases. Ochiai A, Itoh T, Mikami B, Hashimoto W, Murata K. J. Biol. Chem. 284, 10181-9, (2009). View articlePMID: 19193638
Cross References
Representative structure
2zux: Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose