H
IPR036263

Chorismate mutase type II superfamily

InterPro entry
Short nameChorismate_II_sf
Overlapping entries
 

Description

Chorismate mutase (CM) is a regulatory enzyme (
5.4.99.5
) required for biosynthesis of the aromatic amino acids phenylalanine and tyrosine. CM catalyzes the Claisen rearrangement of chorismate to prephenate, which can subsequently be converted to precursors of either L-Phe or L-Tyr. In bifunctional enzymes the CM domain can be fused to a prephenate dehydratase (P-protein for Phe biosynthesis), to a prephenate dehydrogenase (T-protein, for Tyr biosynthesis), or to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase. Besides these prokaryotic bifunctional enzymes, monofunctional CMs occur in prokaryotes as well as in fungi, plants and nematode worms
[1]
. The sequence of monofunctional chorismate mutase aligns well with the N-terminal part of P-proteins
[2]
.

The type II or AroQ class of CM has an all-helical 3D structure, represented by the CM domain of the bifunctional Escherichia coli P-protein. This type is named after the Enterobacter agglomerans monofunctional CM encoded by the aroQ gene
[5]
. All CM domains from bifunctional enzymes as well as most monofunctional CMs belong to this class, including archaeal CM.

Eukaryotic CM from plants and fungi form a separate subclass of AroQ, represented by the Baker's yeast allosteric CM. These enzymes show only partial sequence similarity to the prokaryotic CMs due to insertions of regulatory domains, but the helix-bundle topology and catalytic residues are conserved and the 3D structure of the E. coli CM dimer resembles a yeast CM monomer
[1, 4, 3]
. The E. coli P-protein CM domain consists of 3 helices and lacks allosteric regulation. The yeast CM has evolved by gene duplication and dimerization and each monomer has 12 helices. Yeast CM is allosterically activated by Trp and inhibited by Tyr
[4]
.

References

1.Allosteric regulation of catalytic activity: Escherichia coli aspartate transcarbamoylase versus yeast chorismate mutase. Helmstaedt K, Krappmann S, Braus GH. Microbiol. Mol. Biol. Rev. 65, 404-21, table of contents, (2001). View articlePMID: 11528003

2.Tyrosine and tryptophan act through the same binding site at the dimer interface of yeast chorismate mutase. Schnappauf G, Krappmann S, Braus GH. J. Biol. Chem. 273, 17012-7, (1998). View articlePMID: 9642265

3.A small, thermostable, and monofunctional chorismate mutase from the archaeon Methanococcus jannaschii. MacBeath G, Kast P, Hilvert D. Biochemistry 37, 10062-73, (1998). View articlePMID: 9665711

4.Mechanisms of catalysis and allosteric regulation of yeast chorismate mutase from crystal structures. Strater N, Schnappauf G, Braus G, Lipscomb WN. Structure 5, 1437-52, (1997). View articlePMID: 9384560

5.The aroQ-encoded monofunctional chorismate mutase (CM-F) protein is a periplasmic enzyme in Erwinia herbicola. Xia T, Song J, Zhao G, Aldrich H, Jensen RA. J. Bacteriol. 175, 4729-37, (1993). View articlePMID: 8335631

GO terms

molecular function

  • None

cellular component

  • None

Cross References

Contributing Member Database Entry
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.