H
IPR038508

ArfGAP domain superfamily

InterPro entry
Short nameArfGAP_dom_sf
Overlapping entries
 

Description

Proteins containing this domain include ARF1-directed GTPase-activating protein, the cycle control GTPase activating protein (GAP) GCS1 which is important for the regulation of the ADP ribosylation factor ARF, a member of the Ras superfamily of GTP-binding proteins
[3]
. The GTP-bound form of ARF is essential for the maintenance of normal Golgi morphology, it participates in recruitment of coat proteins which are required for budding and fission of membranes. Before the fusion with an acceptor compartment the membrane must be uncoated. This step required the hydrolysis of GTP associated to ARF. These proteins contain a characteristic zinc finger motif (Cys-x2-Cys-x(16,17)-x2-Cys) which displays some similarity to the C4-type GATA zinc finger. The ARFGAP domain display no obvious similarity to other GAP proteins.

The 3D structure of the ARFGAP domain of the PYK2-associated protein beta has been solved
[2]
. It consists of a three-stranded β-sheet surrounded by 5 α helices. The domain is organised around a central zinc atom which is coordinated by 4 cysteines. The ARFGAP domain is clearly unrelated to the other GAP proteins structures which are exclusively helical. Classical GAP proteins accelerate GTPase activity by supplying an arginine finger to the active site. The crystal structure of ARFGAP bound to ARF revealed that the ARFGAP domain does not supply an arginine to the active site which suggests a more indirect role of the ARFGAP domain in the GTPase hydrolysis
[1]
.

References

1.Structural and functional analysis of the ARF1-ARFGAP complex reveals a role for coatomer in GTP hydrolysis. Goldberg J. Cell 96, 893-902, (1999). View articlePMID: 10102276

2.Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta. Mandiyan V, Andreev J, Schlessinger J, Hubbard SR. EMBO J. 18, 6890-8, (1999). View articlePMID: 10601011

3.Molecular characterization of the GTPase-activating domain of ADP-ribosylation factor domain protein 1 (ARD1). Vitale N, Moss J, Vaughan M. J. Biol. Chem. 273, 2553-60, (1998). View articlePMID: 9446556

Cross References

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