D
IPR041735

4-hydroxyphenylpyruvate dioxygenase, C-terminal

InterPro entry
Short name4OHPhenylPyrv_dOase_C
Overlapping
homologous
superfamilies
 

Description

4-hydroxyphenylpyruvate dioxygenase HppD and 4-hydroxymandelate synthase HmaS are non-heme iron-dependent dioxygenases, which modify a common substrate, 4-hydroxyphenylpyruvate (HPP), but yield different products. HPPD catalyzes the second reaction in tyrosine catabolism, the conversion of HPP to homogentisate (2,5-dihydroxyphenylacetic acid, HG). HmaS converts HPP to 4-hydroxymandelate, a committed step in the formation of hydroxyphenylglycerine, a structural component of nonproteinogenic macrocyclic peptide antibiotics, such as vancomycin
[1, 2]
. If the emphasis is on catalytic chemistry, HPPD and HmaS are classified as members of a large family of alpha-keto acid dependent mononuclear non-heme iron oxygenases most of which require Fe(II), molecular oxygen, and an alpha-keto acid (typically alpha-ketoglutarate) to either oxygenate or oxidize a third substrate. Both enzymes are exceptions in that they require two, instead of three, substrates, do not use alpha-ketoglutarate, and incorporate both atoms of dioxygen into the aromatic product
[3]
. Both HPPD and HmaS exhibit duplicate β barrel topology in their N- and C-terminal domains which share sequence similarity, suggestive of a gene duplication. Each protein has only one catalytic site located in at the C-terminal domain
[4]
. This entry represents the C-terminal domain.

References

1.4-Hydroxyphenylpyruvate dioxygenase. Moran GR. Arch. Biochem. Biophys. 433, 117-28, (2005). View articlePMID: 15581571

2.Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases. Yang C, Pflugrath JW, Camper DL, Foster ML, Pernich DJ, Walsh TA. Biochemistry 43, 10414-23, (2004). View articlePMID: 15301540

3.We two alone will sing: the two-substrate alpha-keto acid-dependent oxygenases. He P, Moran GR. Curr Opin Chem Biol 13, 443-50, (2009). View articlePMID: 19625206

4.Human 4-hydroxyphenylpyruvate dioxygenase. Primary structure and chromosomal localization of the gene. Ruetschi U, Dellsen A, Sahlin P, Stenman G, Rymo L, Lindstedt S. Eur. J. Biochem. 213, 1081-9, (1993). View articlePMID: 8504803

Cross References

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