H
IPR042205

Hepatitis C virus non-structural protein NS2, C-terminal domain

InterPro entry
Short nameHCV_NS2_C
Overlapping entries
 

Description

This superfamily represents the C-terminal domain of hepatitis C virus non-structural protein NS2.

The viral genome is translated into a single polyprotein of about 3000 amino acids. Generation of the mature non-structural proteins relies on the activity of viral proteases. Non-structural protein 2 (NS2) is an zinc-dependent autocatalytic endopeptidase which cleaves at the NS2/NS3 junction
[1, 2]
. The action of NS3 proteinase, which resides in the N-terminal one-third of the NS3 protein, then yields all remaining non-structural proteins.

NS2 belongs to MEROPS peptidase family C18 (hepatitis C virus endopeptidase 2, clan CM). The fold consists of two subdomains connected by an extended linker: an N-terminal helical domain and a C-terminal β sheet. Peptidases are active as a homodimer and the two active sites are formed at the dimer interface by His and Glu from one monomer and Cys from the other. The dimer resembles a 'butterfly' with two-fold symmetry along the vertical axis. The N-terminal subdomain of one molecule interacts with the C-terminal subdomain of the other molecule and vice versa
[3]
.

References

1.The nonstructural proteins of the hepatitis C virus: structure and functions. Neddermann P, Tomei L, Steinkuhler C, Gallinari P, Tramontano A, De Francesco R. Biol. Chem. 378, 469-76, (1997). PMID: 9224925

2.In vitro study of the NS2-3 protease of hepatitis C virus. Pieroni L, Santolini E, Fipaldini C, Pacini L, Migliaccio G, La Monica N. J. Virol. 71, 6373-80, (1997). View articlePMID: 9261354

3.Structure of the catalytic domain of the hepatitis C virus NS2-3 protease. Lorenz IC, Marcotrigiano J, Dentzer TG, Rice CM. Nature 442, 831-5, (2006). View articlePMID: 16862121

Cross References

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