F
IPR042225

Negative cofactor 2 complex subunit beta

InterPro entry
Short nameNcb2
Overlapping
homologous
superfamilies
 
Histone-fold (IPR009072)

Description

This entry includes budding yeast Ncb2 and its homologues. Ncb2 is a component of the NC2 complex which represses RNA polymerase II transcription through binding to Spt15/TBP and thereby inhibiting the assembly of the preinitiation complex
[1]
. Its human homologue, DR1, associates with DRAP1 through histone fold motifs to form the NC2 complex, which negatively regulates transcription initiation
[2]
.

This entry also includes proteins from fungi, such as NctB from Neosartorya fumigata and Ncb2 from Candida albicans. NctB forms a complex with NctA, and this complex acts as a general transcriptional cofactor
[3]
. In Candida albicans, conditional Ncb2 null mutants have been shown to display decreased susceptibility toward azole and an enhanced transcription of CDR1
[4]
.

References

1.Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP). Cang Y, Prelich G. Proc. Natl. Acad. Sci. U.S.A. 99, 12727-32, (2002). View articlePMID: 12237409

2.A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains. Goppelt A, Stelzer G, Lottspeich F, Meisterernst M. EMBO J. 15, 3105-16, (1996). PMID: 8670811

3.The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus. Furukawa T, van Rhijn N, Fraczek M, Gsaller F, Davies E, Carr P, Gago S, Fortune-Grant R, Rahman S, Gilsenan JM, Houlder E, Kowalski CH, Raj S, Paul S, Cook P, Parker JE, Kelly S, Cramer RA, Latge JP, Moye-Rowley S, Bignell E, Bowyer P, Bromley MJ. Nat Commun 11, 427, (2020). PMID: 31969561

4.Ncb2 is involved in activated transcription of CDR1 in azole-resistant clinical isolates of Candida albicans. Shukla S, Yadav V, Mukhopadhyay G, Prasad R. Eukaryotic Cell 10, 1357-66, (2011). PMID: 21856931

GO terms

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