H
IPR043031

Viral ssDNA binding protein, head domain

InterPro entry
Short nameViral_ssDBP_head
Overlapping entries
 

Description

This entry includes the major DNA-binding protein (DBP, UL57 or ICP8) from Herpesviruses. DBP binds single-stranded DNA, and the region encompassing residues 368-902 contains the DNA-binding site
[3]
. UL5, UL8 and UL52 genes encode an essential heterotrimeric DNA helicase-primase that is responsible for concomitant DNA unwinding and primer synthesis at the viral DNA replication fork. DBP may stimulate DNA unwinding and enable bypass of cisplatin damaged DNA by recruiting the helicase-primase to the DNA
[2]
. DBP helps initiate DNA replication by binding to the origin-binding protein (UL9)
[4]
. It also reorganizes the host nucleus leading to the formation of prereplicative sites and replication compartments
[1]
.

This superfamily represents the head domain found in Viral ssDNA-binding protein. The head domain interacts with the C-terminal domain (CTD) of the protein and gives the CTD structure. The CTD is involved in increasing the ssDNA binding protein's cooperativity when binding ICP8, which is believed to stimulate helicase activity. Structurally, this domain consists of 8 α helices
[5]
.

References

1.ICP8 Filament Formation Is Essential for Replication Compartment Formation during Herpes Simplex Virus Infection. Darwish AS, Grady LM, Bai P, Weller SK. J. Virol. 90, 2561-70, (2015). View articlePMID: 26676794

2.Herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) enhances the ability of the viral DNA helicase-primase to unwind cisplatin-modified DNA. Tanguy Le Gac N, Villani G, Boehmer PE. J. Biol. Chem. 273, 13801-7, (1998). View articlePMID: 9593724

3.Photoaffinity labeling of the herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) with oligodeoxyribonucleotides. White EJ, Boehmer PE. Biochem. Biophys. Res. Commun. 264, 493-7, (1999). View articlePMID: 10529391

4.Eleven loci encoding trans-acting factors are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA replication. Pari GS, Anders DG. J. Virol. 67, 6979-88, (1993). PMID: 8230421

5.The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding. Mapelli M, Panjikar S, Tucker PA. J. Biol. Chem. 280, 2990-7, (2005). View articlePMID: 15507432

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