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IPR045747

CRISPR-associated protein Cas6, N-terminal domain superfamily

InterPro entry
Short nameCRISPR-assoc_prot_Cas6_N_sf
Overlapping entries
 

Description

The CRISPR-Cas system is a prokaryotic defense mechanism against foreign genetic elements. The key elements of this defense system are the Cas proteins and the CRISPR RNA.

Cas6 is a member of the RAMP (repeat-associated mysterious protein) superfamily
[1]
. It is among the most widely distributed Cas proteins and is found in both bacteria and archaea
[2]
. Cas6 functions in the generation of CRISPR-derived guide RNAs for invader defense in prokaryotes
[4]
. The structure of this protein showed it adopts a tandem ferredoxin/RRM fold
[3]
.

This superfamily represents the N-terminal domain of Cas6 proteins, which has a ferredoxin-like fold
[4]
.

References

1.A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. Biol. Direct 1, 7, (2006). View articlePMID: 16545108

2.A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. Haft DH, Selengut J, Mongodin EF, Nelson KE. PLoS Comput. Biol. 1, e60, (2005). View articlePMID: 16292354

3.Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases. Niewoehner O, Jinek M, Doudna JA. Nucleic Acids Res 42, 1341-53, (2014). PMID: 24150936

4.Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes. Carte J, Wang R, Li H, Terns RM, Terns MP. Genes Dev. 22, 3489-96, (2008). View articlePMID: 19141480

Further reading

5. Evolutionary conservation of sequence and secondary structures in CRISPR repeats. Kunin V, Sorek R, Hugenholtz P. Genome Biol. 8, R61, (2007). View articlePMID: 17442114

6. CRISPR provides acquired resistance against viruses in prokaryotes. Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P. Science 315, 1709-12, (2007). View articlePMID: 17379808

7. Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein. Howard JA, Delmas S, Ivancic-Bace I, Bolt EL. Biochem. J. 439, 85-95, (2011). View articlePMID: 21699496

8. A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii. Brendel J, Stoll B, Lange SJ, Sharma K, Lenz C, Stachler AE, Maier LK, Richter H, Nickel L, Schmitz RA, Randau L, Allers T, Urlaub H, Backofen R, Marchfelder A. J. Biol. Chem. 289, 7164-77, (2014). View articlePMID: 24459147

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