D
IPR046454

Terminase large subunit GpA, endonuclease domain

InterPro entry
Short nameGpA_endonuclease

Description

This entry consists of several phage terminase large subunit proteins, including GpA (also known as TerL) from Bacteriophage lambda and from Enterobacteria phage P21, as well as related sequences from several bacterial species. The DNA packaging enzyme of Bacteriophage lambda, terminase, is a heteromultimer composed of a small subunit, gpNu1 involved in DNA recognition, and a large subunit, gpA involved in late stages of packaging, products of the Nu1 and A genes, respectively. Terminase is involved in the site-specific binding and cutting of the DNA in the initial stages of packaging. This large subunit is an ATPase required for viral DNA translocation into empty capsids and also acts as an endonuclease that cuts the viral genome from the concetamer to initiate and to end the packaging reaction
[4, 5, 6]
.

This entry represents the C-terminal domain, which shows DNA maturation (nuclease/helicase) catalytic activity
[1, 3, 2]
.

References

1.The enzymology of a viral genome packaging motor is influenced by the assembly state of the motor subunits. Andrews BT, Catalano CE. Biochemistry 51, 9342-53, (2012). PMID: 23134123

2.The DNA maturation domain of gpA, the DNA packaging motor protein of bacteriophage lambda, contains an ATPase site associated with endonuclease activity. Ortega ME, Gaussier H, Catalano CE. J Mol Biol 373, 851-65, (2007). PMID: 17870092

3.Evidence that a catalytic glutamate and an 'Arginine Toggle' act in concert to mediate ATP hydrolysis and mechanochemical coupling in a viral DNA packaging motor. Ortiz D, delToro D, Ordyan M, Pajak J, Sippy J, Catala A, Oh CS, Vu A, Arya G, Feiss M, Smith DE, Catalano CE. Nucleic Acids Res 47, 1404-1415, (2019). PMID: 30541105

4.The large subunit of bacteriophage lambda's terminase plays a role in DNA translocation and packaging termination. Duffy C, Feiss M. J. Mol. Biol. 316, 547-61, (2002). View articlePMID: 11866517

5.Energy-independent helicase activity of a viral genome packaging motor. Chang JR, Andrews BT, Catalano CE. Biochemistry 51, 391-400, (2012). PMID: 22191393

6.Physical and Functional Characterization of a Viral Genome Maturation Complex. Yang TC, Ortiz D, Yang Q, De Angelis RW, Sanyal SJ, Catalano CE. Biophys J 112, 1551-1560, (2017). PMID: 28445747

GO terms

biological process

  • None

cellular component

  • None

Cross References

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