IPR000165
Glucoamylase
InterPro entry
Short name | Glucoamylase |
Overlapping homologous superfamilies | |
family relationships |
Description
This entry represents glucoamylase (GA), also known as glucan 1,4-alpha-glucosidase, which belongs to family 15 (
GH15) in the classification of glycosyl hydrolases.
GA catalyses the release of D-glucose from the non-reducing ends of starch and other oligo- or polysaccharides. Studies of fungal GA have indicated 3 closely-clustered acidic residues that play a role in the catalytic mechanism
[1]. This region is also conserved in a recently sequenced bacterial GA
[2].
The 3D structure of the pseudo-tetrasaccharide acarbose complexed with glucoamylase II(471) from Aspergillus awamori var. X100 has been determined to 2.4A resolution
[3]. The protein belongs to the mainly-α class, and contains 19 helices and 9 strands.
References
1.Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori. Sierks MR, Ford C, Reilly PJ, Svensson B. Protein Eng. 3, 193-8, (1990). View articlePMID: 1970434
2.Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme. Ohnishi H, Kitamura H, Minowa T, Sakai H, Ohta T. Eur. J. Biochem. 207, 413-8, (1992). View articlePMID: 1633799
3.Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution. Aleshin AE, Firsov LM, Honzatko RB. J. Biol. Chem. 269, 15631-9, (1994). View articlePMID: 8195212
GO terms
biological process
molecular function
cellular component
- None
Cross References
Contributing Member Database Entry
- PRINTS:PR00736