F
IPR001719

AP endonuclease 2

InterPro entry
Short nameAP_endonuc_2
Overlapping
homologous
superfamilies
 

Description

AP endonucleases can be classified into two families based on sequence similarity. This family contains members of AP endonuclease family 2.

Family 2 groups the enzymes listed below
[1, 2]
.


 * Bacterial endonuclease IV (
3.1.21.2
).
 * Mycobacterium leprae probable endonuclease
[3]
.
 * Saccharomyces cerevisiae (Baker's yeast) apurinic endonuclease Apn1 (
4.2.99.18
).
 * Caenorhabditis elegans hypothetical protein APN-1 or T05H10.2.


Apn1 and endonuclease IV have been shown to be transition metalloproteins that bind three zinc ions
[4, 5]
. The metal-binding sites have been determined from the 3D-structure of Escherichia coli endonuclease IV
[5, 6, 7]
, which shows an α/β-barrel fold similar to that of other divalent metal-dependent TIM barrel enzymes, such as xylose isomerase.

References

1.Yeast structural gene (APN1) for the major apurinic endonuclease: homology to Escherichia coli endonuclease IV. Popoff SC, Spira AI, Johnson AW, Demple B. Proc. Natl. Acad. Sci. U.S.A. 87, 4193-7, (1990). View articlePMID: 1693433

2.Structure and function of apurinic/apyrimidinic endonucleases. Barzilay G, Hickson ID. Bioessays 17, 713-9, (1995). View articlePMID: 7661852

3.Nucleotide sequence of the first cosmid from the Mycobacterium leprae genome project: structure and function of the Rif-Str regions. Honore N, Bergh S, Chanteau S, Doucet-Populaire F, Eiglmeier K, Garnier T, Georges C, Launois P, Limpaiboon T, Newton S. Mol. Microbiol. 7, 207-14, (1993). View articlePMID: 8446028

4.Metalloenzymes in DNA repair. Escherichia coli endonuclease IV and Saccharomyces cerevisiae Apn1. Levin JD, Shapiro R, Demple B. J. Biol. Chem. 266, 22893-8, (1991). View articlePMID: 1720775

5.Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis. Hosfield DJ, Guan Y, Haas BJ, Cunningham RP, Tainer JA. Cell 98, 397-408, (1999). View articlePMID: 10458614

6.Unraveling the three-metal-ion catalytic mechanism of the DNA repair enzyme endonuclease IV. Ivanov I, Tainer JA, McCammon JA. Proc. Natl. Acad. Sci. U.S.A. 104, 1465-70, (2007). View articlePMID: 17242363

7.DNA apurinic-apyrimidinic site binding and excision by endonuclease IV. Garcin ED, Hosfield DJ, Desai SA, Haas BJ, Bjoras M, Cunningham RP, Tainer JA. Nat. Struct. Mol. Biol. 15, 515-22, (2008). View articlePMID: 18408731

GO terms

biological process

cellular component

  • None

Cross References

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