D
IPR002075

Nuclear transport factor 2 domain

InterPro entry
Short nameNTF2_dom
Overlapping
homologous
superfamilies
 
domain relationships

Description

This entry represent the main structural domain of NTF2, Nuclear RNA export factors and related proteins
[2, 3]
.

Nuclear transport factor 2 (NTF2) is a homodimer which stimulates efficient nuclear import of a cargo protein. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins. NTF2 folds into a cone with a deep hydrophobic cavity, the opening of which is surrounded by several negatively charged residues. RanGDP binds to NTF2 by inserting a conserved phenylalanine residue into the hydrophobic pocket of NTF2 and making electrostatic interactions with the conserved negatively charged residues that surround the cavity
[1]
.

References

1.Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran. Stewart M, Kent HM, McCoy AJ. J. Mol. Biol. 277, 635-46, (1998). View articlePMID: 9533885

2.Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor. Fribourg S, Braun IC, Izaurralde E, Conti E. Mol. Cell 8, 645-56, (2001). View articlePMID: 11583626

3.NTF2 monomer-dimer equilibrium. Chaillan-Huntington C, Butler PJ, Huntington JA, Akin D, Feldherr C, Stewart M. J. Mol. Biol. 314, 465-77, (2001). View articlePMID: 11846560

Further reading

4. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran. Clarkson WD, Corbett AH, Paschal BM, Kent HM, McCoy AJ, Gerace L, Silver PA, Stewart M. J. Mol. Biol. 272, 716-30, (1997). View articlePMID: 9368653

5. Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats. Bayliss R, Leung SW, Baker RP, Quimby BB, Corbett AH, Stewart M. EMBO J. 21, 2843-53, (2002). View articlePMID: 12065398

Cross References

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