D
IPR004939

APC10/DOC domain

InterPro entry
Short nameAPC_su10/DOC_dom
Overlapping
homologous
superfamilies
 
domain relationships

Description

The anaphase-promoting complex (APC) or cyclosome is a multi-subunit E3 protein ubiquitin ligase that regulates important events in mitosis, such as the initiation of anaphase and exit from telophase. The APC, in conjunction with other enzymes, assembles multi-ubiquitin chains on a variety of regulatory proteins, thereby targeting them for proteolysis by the 26S proteasome
[4]
.

One of the subunits of the APC that is required for ubiquitination activity is APC10, a one-domain protein homologous to a sequence element, termed the DOC domain, found in several hypothetical proteins that may also mediate ubiquitination reactions, because they contain combinations of either RING finger (see
[prositedoc:PDOC00449]
), cullin (see
[prositedoc:PDOC00967]
) or HECT (see
[prositedoc:PDOC50237]
) domains
[3, 2, 1]
.

The DOC domain consists of a β-sandwich, in which a five-stranded antiparallel β-sheet is packed on top of a three stranded antiparallel β-sheet, exhibiting a 'jellyroll' fold
[2, 1]
.

Proteins known to contain a DOC domain include:


 * Eucaryotic Doc1/Apc10.
 * Mammalian protein associated with the transcription factor Myc (PAM).
 * Mouse runty-jerky-sterile (RJS) protein.
 * Human HERC2, the ortholog of RJS.

References

1.Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit. Au SW, Leng X, Harper JW, Barford D. J. Mol. Biol. 316, 955-68, (2002). View articlePMID: 11884135

2.Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex. Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P. Nat. Struct. Biol. 8, 784-8, (2001). View articlePMID: 11524682

3.Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV, Schleiffer A, Nasmyth K, Mann M, Peters JM. J. Biol. Chem. 274, 14500-7, (1999). View articlePMID: 10318877

4.Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. Jin L, Williamson A, Banerjee S, Philipp I, Rape M. Cell 133, 653-65, (2008). View articlePMID: 18485873

Cross References

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