F
IPR005907

Glucose-1-phosphate thymidylyltransferase, short form

InterPro entry
Short nameG1P_thy_trans_s
Overlapping
homologous
superfamilies
 

Description

This group of proteins comprises a tightly conserved but broadly distributed subfamily (here designated as short form) of known and putative bacterial glucose-1-phosphate thymidylyltransferases (
2.7.7.24
, also known as dTDP-D-glucose synthase). It is well characterised in several species as the first of four enzymes involved in the biosynthesis of dTDP-L-rhamnose, a cell wall constituent and a feedback inhibitor of the enzyme. The enzyme is active as a homotetramer
[2, 4, 3, 1, 5]
.

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose.

References

1.Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomization. Barton WA, Lesniak J, Biggins JB, Jeffrey PD, Jiang J, Rajashankar KR, Thorson JS, Nikolov DB. Nat. Struct. Biol. 8, 545-51, (2001). View articlePMID: 11373625

2.A structural perspective on the enzymes that convert dTDP-d-glucose into dTDP-l-rhamnose. Dong C, Beis K, Giraud MF, Blankenfeldt W, Allard S, Major LL, Kerr ID, Whitfield C, Naismith JH. Biochem. Soc. Trans. 31, 532-6, (2003). View articlePMID: 12773151

3.Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+. Sivaraman J, Sauve V, Matte A, Cygler M. J. Biol. Chem. 277, 44214-9, (2002). View articlePMID: 12171937

4.Kinetic and crystallographic analyses support a sequential-ordered bi bi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferase. Zuccotti S, Zanardi D, Rosano C, Sturla L, Tonetti M, Bolognesi M. J. Mol. Biol. 313, 831-43, (2001). View articlePMID: 11697907

5.The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (RmlA). Blankenfeldt W, Asuncion M, Lam JS, Naismith JH. EMBO J. 19, 6652-63, (2000). View articlePMID: 11118200

GO terms

biological process

  • None

cellular component

  • None

Cross References

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