F
IPR006197

Peptidase S24, LexA-like

InterPro entry
Short namePeptidase_S24_LexA
Overlapping
homologous
superfamilies
 
family relationships

Description

This signature defines serine peptidases belong to MEROPS peptidase family S24 (LexA family, clan SF). They include:


 * LexA, the repressor of genes in the cellular SOS response to DNA damage
 * MucA and the related UmuD proteins, which are lesion-bypass DNA polymerases, induced in response to mitogenic DNA damage
 * RumA a plasmid encoded homologue of UmuD
[6]

 * RuvA, which is a component of the RuvABC resolvasome that catalyses the resolution of Holliday junctions that arise during genetic recombination and DNA repair
[3]



The LexA, UmuD and MucD proteins interact with RecA, which activates self cleavage either derepressing transcription in the case of LexA
[1]
or activating the lesion-bypass polymerase in the case of UmuD and MucA. UmuD'2, is the homodimeric component of DNA pol V, which is produced from UmuD by RecA-facilitated self-cleavage. The first 24 N-terminal residues of UmuD are removed; UmuD'2 is a DNA lesion bypass polymerase
[1, 2]
. MucA
[5, 4]
, like UmuD, is a plasmid encoded a DNA polymerase (pol RI) which is converted into the active lesion-bypass polymerase by a self-cleavage reaction involving RecA
[7]
.

This group of proteins also contains proteins classified as non-peptidase homologues as they either have been found experimentally to be without peptidase activity, or lack amino acid residues that are believed to be essential for catalytic activity.

References

1.Analysis of Escherichia coli RecA interactions with LexA, lambda CI, and UmuD by site-directed mutagenesis of recA. Mustard JA, Little JW. J. Bacteriol. 182, 1659-70, (2000). View articlePMID: 10692372

2.Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C). Ferentz AE, Walker GC, Wagner G. EMBO J. 20, 4287-98, (2001). View articlePMID: 11483531

3.The RuvABC resolvasome. Dickman MJ, Ingleston SM, Sedelnikova SE, Rafferty JB, Lloyd RG, Grasby JA, Hornby DP. Eur. J. Biochem. 269, 5492-501, (2002). View articlePMID: 12423347

4.Plasmid-encoded MucB protein is a DNA polymerase (pol RI) specialized for lesion bypass in the presence of MucA', RecA, and SSB. Goldsmith M, Sarov-Blat L, Livneh Z. Proc. Natl. Acad. Sci. U.S.A. 97, 11227-31, (2000). View articlePMID: 11016960

5.Intermolecular cleavage by UmuD-like enzymes: identification of residues required for cleavage and substrate specificity. McDonald JP, Peat TS, Levine AS, Woodgate R. J. Mol. Biol. 285, 2199-209, (1999). View articlePMID: 9925794

6.Analysis of the mutagenic properties of the UmuDC, MucAB and RumAB proteins, using a site-specific abasic lesion. Lawrence CW, Borden A, Woodgate R. Mol. Gen. Genet. 251, 493-8, (1996). View articlePMID: 8709953

7.Genetic and biochemical characterization of a novel umuD mutation: insights into a mechanism for UmuD self-cleavage. Sutton MD, Kim M, Walker GC. J. Bacteriol. 183, 347-57, (2001). View articlePMID: 11114935

GO terms

molecular function

cellular component

  • None

Cross References

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